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Research Journal of Environmental Toxicology
  Year: 2011 | Volume: 5 | Issue: 1 | Page No.: 58-64
DOI: 10.3923/rjet.2011.58.64
Alteration in Enzymatic Function of Human Cytochrome P450 by Silver Nanoparticles
W. Warisnoicharoen, P. Hongpiticharoen and S. Lawanprasert

Silver nanoparticles (AgNPs) have been recently used for a wide range of applications including health and household products even though an understanding of their mechanistic action in human is not fully explored. In this study, AgNPs were chemically synthesized, characterized and evaluated for in vitro biological effect on human cytochrome P450 (P450) metabolizing enzymes, namely CYPs 1A2, 2C9, 2C19 and 3A4. The findings indicated that the particle size diameter of AgNPs was 12.42±2.48 nm with negative surface charges, -43.6±0.7 mV. AgNPs could alter the function of P450 by decreasing the enzymatic activities in a concentration dependent manner. AgNPs exhibited the greatest inhibition on CYP3A4 with an IC50 value of 13.52±0.01 μM, followed by CYP2C19 (IC50 = 14.31±0.01 μM), CYP2C9 (IC50 = 26.46±0.01 μM) and CYP1A2 (IC50 = 43.51±0.03 μM), respectively. Thus, an awareness of the exposure to AgNPs is suggested due to their ability to interfere with metabolic pathway in human.
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How to cite this article:

W. Warisnoicharoen, P. Hongpiticharoen and S. Lawanprasert, 2011. Alteration in Enzymatic Function of Human Cytochrome P450 by Silver Nanoparticles. Research Journal of Environmental Toxicology, 5: 58-64.

DOI: 10.3923/rjet.2011.58.64








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