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Research Journal of Environmental Toxicology
  Year: 2011 | Volume: 5 | Issue: 6 | Page No.: 385-391
DOI: 10.3923/rjet.2011.385.391
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Effects of Some Metal Ions on Trout Liver Glucose 6-phosphate Dehydrogenase

M. Cankaya, M. Sisecioglu, M. Ciftci and H. Ozdemir

Information on the mechanism of metal ion inhibition of Glucose 6-phosphate dehydrogenase (G6PD) is limited. The purpose of the present study was to clarify in vitro effect of Hg+2, Cd+2, Pb+2, Fe+2, Cu+2 and Zn+2 ions on the purified glucose 6-phosphate dehydrogenase (G6PD). This enzyme was purified from rainbow trout liver (Oncorhynchus mykiss) by 2’,5’-ADP sepharose 4B affinity gel chromatography. G6PD was purified 1010-fold with a yield of 41.72%. Purity of enzyme checked by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) method and a single band was observed. All of the metal ions caused inhibition of the enzyme activity. Of the metal ions tested, Fe+2 exhibited much higher inhibitory effect at lower concentrations, so it was evidently a more potent inhibitor than the others. Hg+2, Cd+2, Pb+2, Cu+2 and Zn+2 displayed noncompetitive type of inhibition mechanism only Fe+2 exhibited competitive type inhibition. IC50 value of Fe+2, Pb+2, Hg+2, Cu+2, Zn+2 and Cd+2 were 0.39, 0.78, 0.87, 1.19, 1.97, 2.16 and the Ki constants 0.197±0.049, 0.213±0.018, 0.542±0.029, 1.721±0.77, 2.034±0.53 and 2.770±1.21, respectively.
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How to cite this article:

M. Cankaya, M. Sisecioglu, M. Ciftci and H. Ozdemir, 2011. Effects of Some Metal Ions on Trout Liver Glucose 6-phosphate Dehydrogenase. Research Journal of Environmental Toxicology, 5: 385-391.

DOI: 10.3923/rjet.2011.385.391






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