Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Research Journal of Environmental Toxicology
  Year: 2011 | Volume: 5 | Issue: 1 | Page No.: 27-38
DOI: 10.3923/rjet.2011.27.38
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Effect of Sublethal Concentrations of Aqueous Extract of Lepidagathis alopecuroides on Spermatogenesis in the Fresh Water Catfish Clarias gariepinus
E.E. Orlu and U.U. Gabriel

Abstract:
The effect on spermatogenesis of sublethal concentrations of Lepidagathis alopecuroides indiscriminately used for capturing fish was evaluated on fresh water catfish Clarias gariepinus using standard Hematoxylin-eosin histological techniques. Matured testes from 15 adult fish exposed to sublethal concentrations of L. alopecuroides for 21 days were processed by Hematoxylin-eosin histological technique and the effect of the leaf extract on testicular somatic and germ cells of the seminiferous epithelium assessed by light microscopy. Spermatogenesis occurred in cysts and proliferating Spermatogonia B were surrounded by extensions of Sertoli cells. The last mitotic division of the B spermatogonia produced the Preleptotene primary spermatocytes which differentiated in clones through Leptotene, Zygotene, Pachytene to Diplotene, secondary spermatocytes, round spermatids and spermatozoa before spermiation. This sequence of events demonstrates the presence of recognizable cycle of spermiation in Clarias gariepinus. Changes in the germinal epithelium during spermatogenesis showed that sublethal concentrations of this extract induced mitotic inhibition in proliferating spermatogonia populations, inhibited the last mitotic division in B-type spermatogonia and reduced the number of spermatogonia population entering meiotic division. This led to germ cell loss and reduction of primary spermatocytes population, consequently, a decrease in sperm production potential. Exposure of this fish species to sublethal concentrations of Lepidagathis alopecuroides even for short periods induced testis structure alterations and functions including inhibition of spermatogonia A renewal after spermiation. It is thus concluded that freshwater catfish Clarias gariepinus exposed to sublethal concentrations of L. alopecuroides would exhibit reduction in sperm production in the subsequent reproduction cycles.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Contamination of Surface Sediments by Heavy Metals in Ebrié Lagoon (Abidjan, Ivory Coast)
  •    Assessment of Waters Contamination by Nutrients and Heavy Metals in the Ebrie Lagoon (Abidjan, Ivory Coast)
  •    Expression Levels of Hormone Receptor and Vitellogenin mRNAs in Livers of Thai Medaka, Oryzias minutillus, Inhabiting the Suburbs of Bangkok, Thailand
  •    Effect of Sublethal Concentrations of Lepidagathis alopecuroides (Vahl) on Sperm Quality, Fertility and Hatchability in Gravid Clarias gariepinus (Burchell, 1822) Broodstock
  •    Breed and Seasonal Variation in the Testicular Histometric Parameters and Germ Cell Populations of the Barred Plymouth Rock and the Nigerian Indigenous Breeds of the Domestic Fowl (Gallus domesticus)
  •    Daily Sperm Production of the Domestic Fowl (Gallus domesticus) as Determined By Quantitative Testicular Histology and Homogenate Methods
How to cite this article:

E.E. Orlu and U.U. Gabriel, 2011. Effect of Sublethal Concentrations of Aqueous Extract of Lepidagathis alopecuroides on Spermatogenesis in the Fresh Water Catfish Clarias gariepinus. Research Journal of Environmental Toxicology, 5: 27-38.

DOI: 10.3923/rjet.2011.27.38

URL: https://scialert.net/abstract/?doi=rjet.2011.27.38

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 

       

       

Curve Bottom