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Research Journal of Environmental Toxicology

Year: 2009 | Volume: 3 | Issue: 1 | Page No.: 9-23
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Authors

M.M. Osman

S.A. EL-Fiky

Y.M. Soheir

A.I Abeer

Keywords

Research Article

Impact of Water Pollution on Histopathological and Electrophoretic Characters of Oreochromis niloticus Fish

M.M. Osman, S.A. EL-Fiky, Y.M. Soheir and A.I Abeer
The present study suggested in order to explore the capability of two environmental pollutants namely copper sulfate (CuSO4) and lead acetate (CHCOO)3 Pb to induce histopathological changes and changes in the electrophoretic pattern of serum proteins in aquatic organisms. To achieve such a purpose, Oreochromis nilotica were chosen as a test material for the study. After determination of LC50 of both pollutants, fish were treated with 1/5, 1/10 and 1/20 of LC50 of either CuSO4 or (CHCOO)3 Pb for a periods of 2, 4 and 6 weeks. The histopathological studies revealed that both chemicals under study are capable of inducing changes in different fish organs (gills, liver, kidneys and spleen) after 2 and 6 weeks of treatment and at three different concentrations. These histopathological changes were positively correlated in its effects with the increase of pollutants concentration and time of exposure. Concerning the electrophoretic patterns. The control samples exhibited 12 bands with different densities and intensities. The total number of bands were 11, 16 and 5-13 bands after exposure to 1st, 2nd and 3rd concentrations of copper sulfate, respectively. The total number of bands were ranged from 8-17, from 9-13 and from 9-12 bands after exposure to 1st, 2nd and 3rd concentrations of lead acetate respectively.
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