Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Research Journal of Environmental Toxicology
  Year: 2009 | Volume: 3 | Issue: 4 | Page No.: 159-169
DOI: 10.3923/rjet.2009.159.169
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Evaluation of Alcaligenes faecalis Degradation of Chrysene and Diesel Oil with Concomitant Production of Biosurfactant

M.N. Igwo-Ezikpe, O.G. Gbenle, M.O. Ilori, J. Okpuzor and A.A. Osuntoki

Abstract:
Alcaligenes faecalis was evaluated for its potential to degrade varying concentrations of chrysene and diesel oil with concomitant biosurfactant production. Biodegradation was set up for 7 days utilizing the substrates as sole carbon and energy sources. Residual chrysene obtained after degradation of 30, 50 and 100 mg L-1, respectively was 17.4±1.5, 27.2±1.2 and 28.7±1.4 mg L-1 while total petroleum hydrocarbon remaining after degradation of 3, 5, 15 and 30% (v/v) diesel oil respectively was 2.58±0.5, 3.09±1.2, 21.65±5.4 and 63.92±8.1%. Microbial cells of A. faecalis and sterilized cell-free extract from diesel oil media showed emulsifying activities against kerosene, diesel oil, engine oil, hexadecane, dodecane, xylene and hexane whereas no emulsifying activity was observed of microbial cells and sterilized cell-free extract from chrysene media. Alcaligenes faecalis cells harvested from diesel oil media also showed haemolytic activity unlike the microbial cells from chrysene media. Growth of the isolate in chrysene and diesel oil media induced secretion of protein and carbohydrate into the media which were statistically significantly (p<0.05) different compared to controls. This study portrays the potential of Alcaligenes faecalis to degrade and grow on chrysene and diesel oil and induce extracellular protein and carbohydrate with concomitant production of biosurfactant for industrial purposes and in hydrocarbon bioremediation.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Isolation and Characterization of Marine Beneficial Bacteria from Petroleum Contaminated Sites for Better Environment of Animal Life
  •    Isolation and Characterisation of Polycyclic Aromatic Hydrocarbon Degrading Soil Microbes from Automobile Workshop Sediments
  •    Microbial Polycyclic Aromatic Hydrocarbons Degradation in Soil
How to cite this article:

M.N. Igwo-Ezikpe, O.G. Gbenle, M.O. Ilori, J. Okpuzor and A.A. Osuntoki, 2009. Evaluation of Alcaligenes faecalis Degradation of Chrysene and Diesel Oil with Concomitant Production of Biosurfactant. Research Journal of Environmental Toxicology, 3: 159-169.

DOI: 10.3923/rjet.2009.159.169

URL: https://scialert.net/abstract/?doi=rjet.2009.159.169

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 

Curve Bottom