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Research Journal of Environmental Sciences

Year: 2012 | Volume: 6 | Issue: 6 | Page No.: 196-209
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Authors

Hashem Al-Sheikh

Hani M.A. Abdelzaher

Keywords

Research Article

Occurrence, Identification and Pathogenicity of Pythium aphanidermatum, P. diclinum, P. dissotocum and Pythium "Group P" Isolated from Dawmat Al-Jandal Lake, Saudi Arabia

Hashem Al-Sheikh and Hani M.A. Abdelzaher
Pythium species are considered to be an aquatic fungus which occurs much abundantly in aquatic media. There are no sufficient efforts in studying the presence of them in water as compared with study in soil. For this reason, these fungi were studied in the waters of Dawmat Al-Jandal Lake which is essentially not yet attained their roles of biological and environmental studies in this country. In this study, Pythium biota has been investigated in Dawmat Al-Jandal lake, Saudi Arabia in order to clarify a diversity of this important fungus. Pythium species were isolated from water by baiting method with grass blades using Pythium selective medium. Sequencing of the internal transcribed spacer regions of ribosomal DNA (rDNA-ITS) including the 5.8 SrDNA of isolated pythia confirmed identification based on morphological characteristics. Fifty-nine isolates of Pythium spp. were obtained, of these, a total of three species and one group could be identified. Species isolated in the water of the lake were P. aphanidermatum, P. diclinum, sterile P. dissotocum and Pythium 'group P'. P. aphanidermatum was highly pathogenic to tomato seeds causing 100% damping-off but P. diclinum and the sterile isolate of P. dissotocum were moderately pathogenic, while Pythium "group P" was non-pathogenic to tomato seeds causing 60 and 0% damping-off, respectively. It is concluded that Pythium species is present in Dawmat Al-Jandal lake and some of these fungi appeared their virulence to tomato seedlings. It is possible that these pythia represent a risk factor in dissimilation of such microorganisms to the surrounded areas.
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How to cite this article

Hashem Al-Sheikh and Hani M.A. Abdelzaher, 2012. Occurrence, Identification and Pathogenicity of Pythium aphanidermatum, P. diclinum, P. dissotocum and Pythium "Group P" Isolated from Dawmat Al-Jandal Lake, Saudi Arabia. Research Journal of Environmental Sciences, 6: 196-209.

DOI: 10.3923/rjes.2012.196.209

URL: https://scialert.net/abstract/?doi=rjes.2012.196.209

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