
	Research Journal of Botany
	Year: 2011 \| Volume: 6 \| Issue: 1 \| Page No.: 31-37 DOI: 10.3923/rjb.2011.31.37

EMS Induced Mutational Variability in Vigna radiata and Vigna mungo

M. Imran Kozgar, Sonu Goyal and Samiullah Khan

Abstract: Ethylmethane sulphonate (EMS) has been long considered as a potential chemical mutagen for inducing genetic variability in crop plants. This study tries to elucidate out the effect of different doses (0.1 to 0.4%) of EMS on the two species of genus Vigna thereinafter V. radiata and V. mungo in M₁ and M₂ generations for isolation of putative mutants and their respective comparative studies. Selection of mutants is based upon the variation in genetic parameters for yield and certain yield contributing characters. The comparative studies on the estimation of total seed protein content and nitrate reductase (EC 1.7.99.4) activity, NRA, in both generations of these two species have also been studied in order to reveal the potentiality of mutants to be isolated for raising M₃ generation. In the present study, a better variant doses of, EMS were found to be very effective in generating mutation for higher yield in both species and there was a linear correlation of the total seed protein content to NRA and the total plant yield. The accuracy of the selected mutants in M₂ generation was satisfactorily evaluated and would help in isolating wide range of mutant pools accurately to larger extent in shorter duration.

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How to cite this article:

M. Imran Kozgar, Sonu Goyal and Samiullah Khan, 2011. EMS Induced Mutational Variability in Vigna radiata and Vigna mungo. Research Journal of Botany, 6: 31-37.

DOI: 10.3923/rjb.2011.31.37

URL: https://scialert.net/abstract/?doi=rjb.2011.31.37

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