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Research Journal of Botany
  Year: 2007 | Volume: 2 | Issue: 2 | Page No.: 102-107
DOI: 10.3923/rjb.2007.102.107
An Efficient Micropropagation System for Vitex negundo L., an Important Woody Aromatic Medicinal Plant, Through Shoot Tip Culture
P.K. Usha, Sailas Benjamin, K.V. Mohanan and A.V. Raghu

Abstract:
An efficient protocol was established for in vitro shoot multiplication from shoot tip explants of Vitex negundo on Murashige and Skoog (MS) basal medium supplemented with 8.87 μM 6-benzylaminopurine (BA). Inclusion of 8-Naphthalene Acetic Acid (NAA) in the culture medium along with BA promoted higher rates of shoot multiplication than BA alone. The rate shoot multiplication (6.3) after 4 week of culture on MS basal medium supplemented with 8.87 μM BA, 2.69 μM NAA. The elongated shoots rooted in half strength MS basal salts supplemented with 4.90 μM IBA + 2.85 μm IAA and 2% (w/v) sucrose. The presence of Activated Charcoal (AC) with IBA showed positive response to rooting. In vitro propagated plants were transferred to soil with a survival rate of 85% after 1 month.
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How to cite this article:

P.K. Usha, Sailas Benjamin, K.V. Mohanan and A.V. Raghu, 2007. An Efficient Micropropagation System for Vitex negundo L., an Important Woody Aromatic Medicinal Plant, Through Shoot Tip Culture. Research Journal of Botany, 2: 102-107.

DOI: 10.3923/rjb.2007.102.107

URL: https://scialert.net/abstract/?doi=rjb.2007.102.107

 
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