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| Potential Plant Growth-promoting Activity of Pseudomonas spp. and
Bacillus spp. as Biocontrol Agents Against Damping-off in Alfalfa |
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Ehab A.D. Sarhan and Heba Sh. Shehata |
Abstract:
Four strains of plant growth promoting rhizobacteria (Bacillus
subtilis, Paenibacillus polymyxa, Pseudomonas fluorescens
and Pseudomonas putida) and Sinorhizobium meliloti were tested
for their antibiosis toward damping-off disease of growth and yield of the alfalfa
crop during 2010-2011 and 2011-2012 seasons. In vitro the four PGPR strains
produced hydrogen cyanide (HCN), indole-3-acetic acid (IAA), siderophore, solubilized
insoluble phosphate and showed protease and β-1,3-glucanase activities,
whereas S. meliloti produced IAA and solubilized insoluble phosphate
only. PGPR strains strongly inhibited growth of Fusarium oxysporum,
Fusarium solani and Rhizoctonia solani in vitro. Furthermore,
Ps. fluorescens was the most effective followed by P. polymyxa, B.
subtilis and Ps. putida respectively. Seed treatment with the tested
PGPR strains significantly reduced alfalfa damping-off diseases incidence compared
to untreated control under greenhouse and field conditions. Ps. fluorescens
and P. polymyxa combined with S. meliloti recorded the highest
reduction in diseases incidence. Under greenhouse conditions, uninoculated plant
recorded the lowest nodule number and biomass, while co-inoculation with S.
meliloti and mixture of the four tested strains recorded the highest values.
Same results were obtained when alfalfa seeds were treated by PGPR combined
with S. meliloti on Number of nodules, dry weights of nodules, as well
as plant height, tillers/m2 fresh, dry weight and protein content
of alfalfa plants under field conditions. Ps. fluorescens and P. polymyxa
combined with S. meliloti recorded the highest values followed by B.
subtilis and Ps. putida respectively.
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How to cite this article:
Ehab A.D. Sarhan and Heba Sh. Shehata, 2014. Potential Plant Growth-promoting Activity of Pseudomonas spp. and
Bacillus spp. as Biocontrol Agents Against Damping-off in Alfalfa. Plant Pathology Journal, 13: 8-17.
DOI: 10.3923/ppj.2014.8.17
URL: https://scialert.net/abstract/?doi=ppj.2014.8.17
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| COMMENTS |
03 April, 2014
Dr.Mohamed Fathy Salem:
Dear Dr.Ehab
I would like to appreciate your recent paper and this trend of biocontrol through PGPR. We need the application of this studies in newly reclaimed areas in Egypt and developing countries.
Regards
Mohamed Salem |
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