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Plant Pathology Journal
  Year: 2014 | Volume: 13 | Issue: 1 | Page No.: 8-17
DOI: 10.3923/ppj.2014.8.17
Potential Plant Growth-promoting Activity of Pseudomonas spp. and Bacillus spp. as Biocontrol Agents Against Damping-off in Alfalfa
Ehab A.D. Sarhan and Heba Sh. Shehata

Abstract:
Four strains of plant growth promoting rhizobacteria (Bacillus subtilis, Paenibacillus polymyxa, Pseudomonas fluorescens and Pseudomonas putida) and Sinorhizobium meliloti were tested for their antibiosis toward damping-off disease of growth and yield of the alfalfa crop during 2010-2011 and 2011-2012 seasons. In vitro the four PGPR strains produced hydrogen cyanide (HCN), indole-3-acetic acid (IAA), siderophore, solubilized insoluble phosphate and showed protease and β-1,3-glucanase activities, whereas S. meliloti produced IAA and solubilized insoluble phosphate only. PGPR strains strongly inhibited growth of Fusarium oxysporum, Fusarium solani and Rhizoctonia solani in vitro. Furthermore, Ps. fluorescens was the most effective followed by P. polymyxa, B. subtilis and Ps. putida respectively. Seed treatment with the tested PGPR strains significantly reduced alfalfa damping-off diseases incidence compared to untreated control under greenhouse and field conditions. Ps. fluorescens and P. polymyxa combined with S. meliloti recorded the highest reduction in diseases incidence. Under greenhouse conditions, uninoculated plant recorded the lowest nodule number and biomass, while co-inoculation with S. meliloti and mixture of the four tested strains recorded the highest values. Same results were obtained when alfalfa seeds were treated by PGPR combined with S. meliloti on Number of nodules, dry weights of nodules, as well as plant height, tillers/m2 fresh, dry weight and protein content of alfalfa plants under field conditions. Ps. fluorescens and P. polymyxa combined with S. meliloti recorded the highest values followed by B. subtilis and Ps. putida respectively.
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How to cite this article:

Ehab A.D. Sarhan and Heba Sh. Shehata, 2014. Potential Plant Growth-promoting Activity of Pseudomonas spp. and Bacillus spp. as Biocontrol Agents Against Damping-off in Alfalfa. Plant Pathology Journal, 13: 8-17.

DOI: 10.3923/ppj.2014.8.17

URL: https://scialert.net/abstract/?doi=ppj.2014.8.17

COMMENTS
03 April, 2014
Dr.Mohamed Fathy Salem:
Dear Dr.Ehab
I would like to appreciate your recent paper and this trend of biocontrol through PGPR. We need the application of this studies in newly reclaimed areas in Egypt and developing countries.
Regards
Mohamed Salem
 
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