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Plant Pathology Journal
  Year: 2010 | Volume: 9 | Issue: 4 | Page No.: 179-187
DOI: 10.3923/ppj.2010.179.187
Physiological Study and both in vitro and in vivo Antifungal Activities against Stemphylium botryosum causing Stemphylium Blight Disease in Lentil (Lens culinaris)
Tanjina Rahman, A.U. Ahmed, M.R. Islam and M.I. Hosen

Abstract:
The experiment was carried out to study and the evaluation of six fungicides and an antagonist both in vitro and in vivo condition against stemphylium blight disease of lentil caused by Stemphylium botryosum. The investigation was undertaken at Plant Pathology Division, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh during September 2007 to April 2008 using completely randomized design in lab condition and completely randomized block design in filed condition. The colony color of S. botryosum was greenish brown, irregular margin and velvety texture was found on PDA medium. The suitable temperature and pH for the maximum colony growth was recorded at 25°C and 6.0, respectively. The maximum (100%) germination of conidia was found at 25°C after 6 h of incubation. From the in vitro test, six fungicides have the potentiality to inhibit the radial mycelial growth at a lower (500 ppm) concentration except Agrimyl (Mancozeb+Metalaxyl) whereas it inhibited radial colony growth at higher (2000 ppm) concentration. In field condition the minimum disease score (1.0) was recorded from Iprosan 50 WP treated plot and the highest (4.75) was found in control plot. Among the six fungicides Iprosan from the iprodione group gave the best performance. The highest root length (9.48 cm), shoot length (44.6 cm), number of branches plant-1 (9.25), number of pods plant-1 (39.10), thousand grain weight (21.08 g) and grain yield (1271.00 kg ha-1) was obtained from Iprosan 50 WP.
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How to cite this article:

Tanjina Rahman, A.U. Ahmed, M.R. Islam and M.I. Hosen, 2010. Physiological Study and both in vitro and in vivo Antifungal Activities against Stemphylium botryosum causing Stemphylium Blight Disease in Lentil (Lens culinaris). Plant Pathology Journal, 9: 179-187.

DOI: 10.3923/ppj.2010.179.187

URL: https://scialert.net/abstract/?doi=ppj.2010.179.187

 
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