For evaluation yield and Verticillium resistance in cotton, 21 genotypes were screened as Randomized Complete Block Design (RCBD) with 4 replications. Disease percent, index and severity were determinated after harvesting stage. Results showed that Sahel and smooth leaf had the most disease percent (89.56 and 92.56%, respectively) and these genotypes had the greatest infected plant. On the other hand, Sahel had the highest severity and Q29 cultivar had the lowest disease severity among test genotypes. Sahel, Smooth leaf and Gokroba had the most disease index and Q29 and Termez14 had the least disease index.