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Pakistan Journal of Nutrition
  Year: 2019 | Volume: 18 | Issue: 5 | Page No.: 443-454
DOI: 10.3923/pjn.2019.443.454
 
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Characteristics of Gelatin Extracted from Indonesian Local Cattle Hides Using Acid and Base Curing
Dedes Amertaningtyas, Zaenal Bachrudin , Jamhari , Koo Bok Chin and Yuny Erwanto

Abstract:
Background and Objective: Gelatin quality is affected by factors including the animal source materials, the age of the animal and curing and extraction processes. However, the effects of the cattle breed on gelatin quality have not been reported. This study aimed to determine the characteristics of gelatin from Indonesian local cattle [Bali, Madura and Ongole Crossbred (OC)] hides with acid and base curing. Materials and Methods: The hides were treated with 0.25 M hydrochloric acid and 0.25 M sodium hydroxide. The data were analyzed using a completely randomized design with a nested pattern and three replicates. The mean differences in the data were analyzed using Duncan’s multiple range test. Results: The highest gelatin yield was 11.04%, the pH was 9.91, the protein content was 83.45%, the soluble protein content was 12.82 mg mL1, the viscosity was 8 cP and the gel strength was 166 Bloom. The Aw, moisture content, fat content and color values of the gelatins did not differ significantly (p>0.05) among the cattle breeds or between the curing treatments. The molecular weight determinations of the HCl-derived gelatin showed clearer bands than those from NaOH, including showing a band for low-molecular weight proteins (size range between 10 and 25 kDa). Fourier transform infrared (FTIR) spectroscopy showed that while the absorbance intensities of the O-H, C-H, C ≡ C, C ≡ N, C = C, C-C and C-O functional groups from the gelatin samples varied, the peaks were still indicative of the functional groups typically present in gelatin. The most abundant amino acid was glycine at 48.73 g/100 g, followed by glutamic acid at 18.69 g/100 g and arginine at 14.77 g/100 g and histidine was not detected. Conclusion: The use of 0.25 M HCl was more effective and efficient than using 0.25 M NaOH. The amino acid content from the OC hide treated with NaOH was higher than those obtained with the other treatments.
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How to cite this article:

Dedes Amertaningtyas, Zaenal Bachrudin, Jamhari , Koo Bok Chin and Yuny Erwanto, 2019. Characteristics of Gelatin Extracted from Indonesian Local Cattle Hides Using Acid and Base Curing. Pakistan Journal of Nutrition, 18: 443-454.

DOI: 10.3923/pjn.2019.443.454

URL: https://scialert.net/abstract/?doi=pjn.2019.443.454

 
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