Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Pakistan Journal of Nutrition
  Year: 2019 | Volume: 18 | Issue: 4 | Page No.: 324-332
DOI: 10.3923/pjn.2019.324.332
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Characterization of the Acid- and Pepsin-Soluble Collagens from Haruan (Channa striatus) Scales
Bagus Fajar Pamungkas, Supriyadi , Agnes Murdiati and Retno Indrati

Abstract:
Background and Objective: The optimal use of haruan scales for preparing collagen is a promising method to increase value-added products and to protect the environment. The aim of this research was to characterize the acid- and pepsin-soluble collagens from haruan (Channa striatus) scales. Materials and Methods: The fish scales were subjected to an extraction with 0.5 M acetic acid and a digestion with 0.1% pepsin. The results are presented as the mean±standard deviation. Results: The yields of the acid-soluble collagen in the haruan scales (ASC-SH) and the pepsin-soluble collagen in the haruan scales (PSC-SH) were 1.44 and 2.94% (on a dry basis), respectively. ASC-SH and PSC-SH contained glycine as the major amino acid and had high imino acid contents (238 and 242 residues/1,000 residues, respectively). Based on the SDS-PAGE pattern, both ASC-SH and PSC-SH were identified as type I collagens containing α1 and α2 chains. β and γ components were also found in both collagens. The FTIR spectra indicated that both collagens had triple helical structures. The collagens were both soluble at acidic pH levels (1-4) and their solubility was low when the NaCl concentrations were above 3% (w/v). Conclusion: It was concluded that haruan scales could be an alternative source of collagen and that the characteristics of the collagens were slightly affected by the extraction process used in this study.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

Bagus Fajar Pamungkas, Supriyadi , Agnes Murdiati and Retno Indrati, 2019. Characterization of the Acid- and Pepsin-Soluble Collagens from Haruan (Channa striatus) Scales. Pakistan Journal of Nutrition, 18: 324-332.

DOI: 10.3923/pjn.2019.324.332

URL: https://scialert.net/abstract/?doi=pjn.2019.324.332

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

       

       

Curve Bottom