Background and Objective: Coleus amboinicus L., also known asbangun-bangun in Indonesia, is a herb that is often used to increase digestibility and milk production, facilitate metabolic processes in the body and increase VFA. This study aimed to determine the effect of Coleus amboinicus L. supplementation on in vitro digestibility. Methodology: The study used a completely randomized design with 4 treatments and 4 replications: B0 = control (70% forage+30% concentrate), B1 = B0+0.3 g kg1, B2+0.6 g kg1 and B3 = B0+0.9 g kg1 supplementation with C. amboinicus. The observed variables were dry matter digestibility (DMD), organic matter digestibility (OMD), acid detergent fiber (ADF), neutral detergent fiber (NDF), ammonia (NH3), pH, volatile fatty acid (VFA), acetic acid but yric acid, propionic acid, isobutyric acid, valeric acid, isovaleric acid, bacterial count and rumen protozoa. Results: The results demonstrated that supplementation with C. amboinicus significantly increased DMD, especially in treatment B1 (74.62±11.30%) and B2 (74.79±6.45%) and increased OMD in B1 (77.74±10.80%) and B2 (78.26±7.76%). However, supplementation with C. amboinicus did not affect ADF, NDF, In vitro gas production, pH of rumen, valeric acid, isovaleric acid, isobutyric acid, bacterial count or rumen protozoa amount. Supplementation with C. amboinicus significantly increased N-NH3 (33.19±1.54 nM), VFA (106.39±6.20 mM), acetic acid (59.49±1.94 mM), propionic acid (26.50±2.70 mM) and butyric acid (11.49±1.88 mM) in treatment B1. Conclusion: Supplementation with C. amboinicus may increase DMD, OMD, VFA, acetic acid, propionic acid and butyric acid but does not affect ADF, NDF, pH, gas production, isobutyric acid, valeric acid, isovaleric acid, bacterial count or rumen protozoa.