Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Pakistan Journal of Nutrition
  Year: 2019 | Volume: 18 | Issue: 2 | Page No.: 125-133
DOI: 10.3923/pjn.2019.125.133
Postprandial In vitro Protease-Specific Activity of Nile Tilapia (Oreochromis niloticus L.) Digestive Organs
Thatsanee Anukoolprasert, Khajornkiat Srinuansom, Thanasorn Rukdontri, Sataphon Nonkhukhetkhong and Rakpong Petkam

Abstract:
Background and Objective: Protease-specific activity varies postprandially. This study aimed to identify the optimum time after feeding Nile tilapia (Oreochromis niloticus L.) to collect digestive organ samples (stomach, proximal intestine, distal intestine and liver) to obtain maximum protease-specific activity values. Materials and Methods: One hundred Nile tilapia (average weight 55.19±1.78 g/fish) were acclimatized and fed two times per day for 14 days. At the beginning of the experiment, 6 fish were randomly taken from the tank before feeding (0 h) and then 6 fish were also randomly taken after feeding at 1, 3, 6, 12, 18, 24, 36 and 48 h for digestive organ collection. Homogenized organ pH was measured and extracted for a protease-specific activity assay using azocasein as a substrate. Results: The pH values of all organs differed (p<0.05) after feeding and the pH changes were related to chyme movement in these organs. The protease-specific activities of the stomach and proximal intestine were highest at 24 h after feeding (p<0.05) but the protease-specific activities of the distal intestine and liver were not significantly different (p>0.05) between time points. Conclusion: Postprandial digestive organ sample collection was recommended at 24 h to maximize protease-specific activity for further protease characterization.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

Thatsanee Anukoolprasert, Khajornkiat Srinuansom, Thanasorn Rukdontri, Sataphon Nonkhukhetkhong and Rakpong Petkam, 2019. Postprandial In vitro Protease-Specific Activity of Nile Tilapia (Oreochromis niloticus L.) Digestive Organs. Pakistan Journal of Nutrition, 18: 125-133.

DOI: 10.3923/pjn.2019.125.133

URL: https://scialert.net/abstract/?doi=pjn.2019.125.133

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

       

       

Curve Bottom