Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Pakistan Journal of Nutrition
  Year: 2018 | Volume: 17 | Issue: 8 | Page No.: 361-367
DOI: 10.3923/pjn.2018.361.367
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Isolation and Characterization of Lactic Acid Bacteria Proteases from Bekasam for use as a Beef Tenderizer

Afriani , Arnim , Yetti Marlida and Yuherman

Abstract:
Background and Objective: Proteases are important enzymes and have high economic value due to their wide applications in the food industry as a meat tenderizer. Protease use in the food industry necessitates an understanding of the capabilities and influencing factors of these enzymes to accelerate enzymatic reactions. This study aimed to isolate and characterize the proteases of lactic acid bacteria (LAB) from Bekasam. Methodology: The samples were obtained from the third, fifth, seventh, ninth and eleventh day of fermentation to isolate the proteolytic LAB. Characterization of proteases includes the incubation time, casein substrate concentration, optimum temperature and pH, metal ion contents and stability. The LAB with the highest protease activity is identified molecularly and isolated through 16S rDNA sequencing and phylogenetic analysis based on the Neighbor Joining method. Results: The results showed that the best isolate was BAF-715 because it had the highest protease activity (18.84 U mL–1) at 40 h of incubation. The optimum activity of this protease on a casein substrate at 2.5% occurred at an incubation temperature of 40°C at pH 7 and in the presence of Mg2+ and Mn2+ (5 mM) as activators. Based on molecular DNA identification, the BAF-715 isolate is determined to be Pediococcus pentosaceus. Conclusion: A protease produced by Pediococcus pentosaceus showed the highest proteolytic activity, making it the best protease for application as a beef tenderizer.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

Afriani , Arnim , Yetti Marlida and Yuherman , 2018. Isolation and Characterization of Lactic Acid Bacteria Proteases from Bekasam for use as a Beef Tenderizer. Pakistan Journal of Nutrition, 17: 361-367.

DOI: 10.3923/pjn.2018.361.367

URL: https://scialert.net/abstract/?doi=pjn.2018.361.367

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 

Curve Bottom