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Pakistan Journal of Nutrition
  Year: 2015 | Volume: 14 | Issue: 8 | Page No.: 517-523
DOI: 10.3923/pjn.2015.517.523
 
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Isolation, Purification and Characterization of Lactoferrin from Goat Colostrum Whey
Zainab H. Abbas, Kifah S. Doosh and Nahi Y. Yaseen

Abstract:
Lactoferrin is an important protein in many biological applications as a potential cancer treatment agent. In this study, lactoferrin was purified from goat colostrum by ion exchange chromatography through CM-Sephadex C-50 column and gel filtration chromatography through Sephadex G-200 column. The purification fold and yield were (20.83 time and 62.50%) respectively. The purity of lactoferrin to homogeneity was examined by polyacrylamide gel electrophoresis under denaturing conditions (SDS-PAGE) with single bond. Some biochemical characteristics of the purified Lactoferrin were determined, The molecular weight of LF were 80 and 79.50kDa as determined by gel filtration and SDS-PAGE respectively . The percentage of carbohydrate content in goat Lactoferrin was 10.4%, mean while the Iron percentage was 123 ppm and as a saturated percent 7.8%.
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How to cite this article:

Zainab H. Abbas, Kifah S. Doosh and Nahi Y. Yaseen, 2015. Isolation, Purification and Characterization of Lactoferrin from Goat Colostrum Whey. Pakistan Journal of Nutrition, 14: 517-523.

DOI: 10.3923/pjn.2015.517.523

URL: https://scialert.net/abstract/?doi=pjn.2015.517.523

COMMENTS
19 September, 2015
Dr Ali Husain:
Actually this is very interesting paper which enlijghtens the role of goat milk in cancer treatment. Good luck for the authors
 
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