An experiment was conducted at the Department of Animal Resources poultry farm, College of Agriculture, University of Basra, from 27/11/2009-7/1/2010. The study aimed to determine the effect of water quality aggregated from different location from Basra province on broilers performance. A total of 120 day-old chicks were randomly distributed into four treatments with three replicated of ten chicks were given T1 University tap water (Albdai, Thi-Qar), T2 R.O water, T3 Garmat Ali raw river water (North of Basra), T4 Abou-Al-Kasseb tap water (South of Basra). pH, Salinity, Alkalinity, TDS and EC were analysied to those kinds of water. Increase in TDS (>3.086, 5.936 mg/L and salinity) water (>3.60, 4.33) in T3 and T4 caused decrease body weight, hematological traits, tibia length and production index and an increase in feed and water intake, feed conversation ratio, faecal moisture percentage; serum cholesterol, total protein, albumin, globulin, albumin globulin ratio, urea, AST, ALT, Sodium, potassium, chloride, tibia ash percentage and some relative weight organs. Decreasing level TDS and salinity (<0.165, 0.3, R.O. T2) has also deteriorate some of the above traits but not as much an in T3 and T4. Therefore, a level of (1.385, T1) TDS and salinity (1.08, T1) are essential for broiler normal performance, hematological; serum biochemical traits and electrolyte levels which reflected in perfect jejunum histology and production index.