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Pakistan Journal of Nutrition
  Year: 2005 | Volume: 4 | Issue: 2 | Page No.: 101-105
DOI: 10.3923/pjn.2005.101.105
 
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Extraction, Purification, and Inhibitory Effect of Alpha - Amylase Inhibitor from Wheat (Triticum aestivum Var. Zarrin)
R. Heidari , S. Zareae and M. Heidarizadeh

Abstract:
Plant alpha-amylase inhibitors show great potential as tools to engineer resistance of crop plants against pests. They are also drug-design targets for treatment of diabetes and digestion disorderes. These inhibitors also known as sensitizing agents in human. The numerous form of alpha-amylase inhibitors was reported. In this study alpha-amylase inhibitor was extracted from Iranian wheat cultivar (Triticum aestivum v zarrin), precipitated and purified by anion exchange fast protein liquid chromatography. Electrophoresis of purified protein showed 0.66 relative mobility. Total hydrolytic activity of human salivary and bacillus subtilis alpha-amylase were inhibited 97.07% and 89.97% respectively by collected purified alpha-amylase inhibitor.
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How to cite this article:

R. Heidari , S. Zareae and M. Heidarizadeh , 2005. Extraction, Purification, and Inhibitory Effect of Alpha - Amylase Inhibitor from Wheat (Triticum aestivum Var. Zarrin). Pakistan Journal of Nutrition, 4: 101-105.

DOI: 10.3923/pjn.2005.101.105

URL: https://scialert.net/abstract/?doi=pjn.2005.101.105

 
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