Background and Objective:Diabetes mellitus is characterized by hyperglycemia caused by disturbances in pancreatic β cells resulting in the formation of reactive oxygen species which in this study was characterized by increased levels of nitric oxide and malondialdehyde in male Wistar rats with diabetes mellitus models. Materials and Methods: Animal modeling of diabetes mellitus using 40 mg kg1 b.wt. streptozotocin intraperitoneally. The antioxidant activity is based on the ferric reducing antioxidant power (FRAP) method. Determination of nitric oxide and MDA levels using the Griess method and Thiobarbituric Acid Reactive Substances (TBARS) Assay, respectively. The animal models were divided into six treatment groups, normal control (KN), positive control (K+) (glibenclamide), negative control (K) (Na-CMC 0.5%), teak leaf ethanol extract group at a dose of D1 (100 mg kg1 b.wt.), D2 (200 mg kg1 b.wt.) and D3 (300 mg kg1 b.wt.). Results: The antioxidant activity showed that the IC50 values of vitamin C and the ethanol extract of Tectona grandis leaves were 18.208 and 62.236 μg mL1, respectively. The NO levels in KN = 83.133 μmol L1, K+ = 118.300 μmol L1, K = 317.467 μmol L1, D1 = 210.133 μmol L1, D2 = 184.467 μmol L1 and D3 = 129.300 μmol L1. The MDA levels at KN = 3.767 μmol L1, K+ = 8.854 μmol L1, K = 31.032 μmol L1, D1 = 27.010 μmol L1, D2 = 20.166 μmol L1 and D3 = 15.512 μmol L1. Conclusion: The ethanol extract of teak leaves Tectona grandis L. at a dose of 300 mg kg1 reduces nitric oxide levels and plasma malondialdehyde levels in rats through the activity of antioxidant compounds. PDFFulltextXMLReferencesCitation
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Parawansah, Nuralifah, Ruslin, Nurramadhani Armada Sida, Fadhliyah Malik and Atymutmain, 2023. Effects of Tectona grandis L. Extract in Diabetic Rats on Nitric Oxide and Malondialdehyde Levels. Pakistan Journal of Biological Sciences, 26: 177-184.