Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Pakistan Journal of Biological Sciences
  Year: 2021 | Volume: 24 | Issue: 4 | Page No.: 445-453
DOI: 10.3923/pjbs.2021.445.453
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Production of Zinc and Copper as Nanoparticles by Green Synthesis Using Pseudomonas fluorescens

Mohamed Hesham Fawzy, Sara Mohamed Mahmoud, Mohamed Ahmed Hanafy, Mohamed Hassan Bakr, Adel Eid Mohamed Mahmoud , Mohamed Abdel-Alim Ali and Olfat Sayed Barakat

Abstract:
Background and Objective: Nanoparticles with a little size to an enormous surface (1-100 nm) have expected clinical, mechanical and agricultural applications. This study aimed to produce nano Zinc Oxide (ZnO) and nano Copper Oxide (CuO) particles by green synthesis. Materials and Methods: Two strains of Pseudomonas fluorescens i.e., PSI and PSII, both cell culture supernatants and cell pellets from the two strains were examined separately in CuSO4 or ZnSO4 solutions. The supernatants from both strains produced color changes in both solutions referring to the formation of nano CuO or ZnO particles. The solutions were examined for nano-particle characteristics using UV-spectroscopy, particle size and morphology were tested using a scanning electron microscope and transmission electron microscopy. Results: UV-Vis absorption spectrum of solutions at a wavelength range 200-800 nm exhibits a distinct absorption peak in the region of 238-331 and at 303-366 nm for CuO or ZnO NPs, respectively. Absorption bands and the characteristic Surface Plasmon Resonance (SPR) spectra confirm the existence of CuO and ZnO NPs. SEM analysis micrographs indicated that CuO NPs were formed as spherical particles, while the exact shape of ZnO NPs could be identified as oval aggregates. Conclusion: Changes of color occurred in both solutions of two strains referring to the formation of nano CuO or ZnO particles.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

Mohamed Hesham Fawzy, Sara Mohamed Mahmoud, Mohamed Ahmed Hanafy, Mohamed Hassan Bakr, Adel Eid Mohamed Mahmoud, Mohamed Abdel-Alim Ali and Olfat Sayed Barakat, 2021. Production of Zinc and Copper as Nanoparticles by Green Synthesis Using Pseudomonas fluorescens. Pakistan Journal of Biological Sciences, 24: 445-453.

DOI: 10.3923/pjbs.2021.445.453

URL: https://scialert.net/abstract/?doi=pjbs.2021.445.453

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 

Curve Bottom