Subscribe Now Subscribe Today
Science Alert
Curve Top
Pakistan Journal of Biological Sciences
  Year: 2019 | Volume: 22 | Issue: 6 | Page No.: 299-308
DOI: 10.3923/pjbs.2019.299.308
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Protective Role of Carissa edulis Ethanolic Extract Against Dimethoate-induced Hepatotoxicity in Guinea Pigs

Yahya Saleh Al-Awthan and Omar Salem Bahattab

Background and Objective: Carissa edulis (CE) (Apocynaceae) is distributed in tropical Africa and Asia and commonly used in folk medicine to treat many diseases such as headache, cough, rheumatism and fever. The purpose of this study was to evaluate the protective role of ethanolic extract of CE, a medicinal plant locally called "Al-Arm" in Yemen, against liver injury induced by dimethoate (DM) intoxication in male guinea pigs. Materials and Methods: Animals were divided randomly into 5 groups and kept at 5 animals per group. The first group was served as a control group and administered with vehicle orally; the group II administered with DM (14 mg kg1; 1/25 LD50) orally. Animals of group III, IV and V were administered with 100 mg kg1 of CE extract, 200 mg kg1 of CE extract and 100 mg kg1 Liv-52 orally half hour before DM administration, respectively. All the previous administrations were repeated daily for 21 days. Data were analyzed by one-way ANOVA using SPSS. Results: The DM caused a statistically significant increase in the serum level of liver enzymes (AST, ALT, ALP) when compared to control animals, whereas CE and Liv-52 pre-treatment to the DM-intoxicated animals resulted in a significant normalization of the activities of enzymes. Similarly, a significant increase in lipid peroxidation (LPO) level, while induced significant decreases in the activities of liver catalase (CAT) and glutathione-S-transferase (GST). In contrast, co-administration of CE and Liv-52 to DM-treated animals restored most of these biochemical parameters to nearly normal levels. Histopathological examination of intoxicated animals showed many tissues alterations such as; vasodilation, hemorrhage, cytoplasmic vacuolization, inflammation and nuclear pyknosis indicating liver damage, while the animals received CE or Liv-52 showed less pathological effects when compared to animals treated with DM alone. Conclusion: The biochemical and histological results confirmed the hepatoprotective effect of ethanolic extract of CE against DM-induced hepatotoxicity in male guinea pigs.
PDF Fulltext XML References Citation Report Citation
  •    Protective Role of Achillea biebersteinii Pretreatment on Dimethoate Induced Oxidative Stress in Guinea Pigs Liver
  •    Effects of Aqueous Extract of Withania somnifera on Some Liver Biochemical and Histopathological Parameters in Male Guinea Pigs
How to cite this article:

Yahya Saleh Al-Awthan and Omar Salem Bahattab, 2019. Protective Role of Carissa edulis Ethanolic Extract Against Dimethoate-induced Hepatotoxicity in Guinea Pigs. Pakistan Journal of Biological Sciences, 22: 299-308.

DOI: 10.3923/pjbs.2019.299.308






Curve Bottom