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Pakistan Journal of Biological Sciences
  Year: 2015 | Volume: 18 | Issue: 6 | Page No.: 273-278
DOI: 10.3923/pjbs.2015.273.278
Isolation and Characterization of Aedes aegypti Microsatellite Markers Dengue Hemorrhagic Fever Vector in West Sumatra, Indonesia
Hasmiwati , Jamsari , Yanwirasti , Nuzulia Irawati and Dahelmi

Abstract:
Aedes aegypti is the major vector of DHF virus in the tropical and subtropical. The DHF prevention depends on vector control because the vaccine is still in development. Microsatellite has become on effective marker to obtain information about genetic diversity and analyze the structure of genetic population. The aim of this study was to isolate and characterize A. aegypti microsatellite markers, the Dengue Hemorrhagic Fever (DHF) vector in West Sumatra. Sequences containing microsatellites were obtained by enrichment method. Stages of works were as follows: isolation of A. aegypti genomic DNA, restriction with enzymes, ligation with adapters and hybridization by using microsatellite motifs. Furthermore, the candidate fragment contained motifs cloned on plasmid pGEM T easy vector using E. coli DH 5α with blue-white colony screening. The results showed 46 clones were successfully extracted from a total of 152 clones and became microsatellite motifs with repetition: (GA)3, (CTA)3, (GA)3 (TAAG)3, (ACTT)3 (TC)3 (AC)3. Eight pairs of primer were successfully designed from sequences containing microsatellite motifs with feasible flanking regions. The primer evaluation used 32 DNA samples of A. aegypti from 8 cities (population) in West Sumatra. These markers have been successfully amplified 9-17 alleles with amplification products ranging from 129-306 bp, with a high degree of polymorphism. Aedes aegypti microsatellite markers obtained can be used to analyze the structure of genetic population of A. aegypti and the obtained results were the additional microsatellite markers type of A. aegypti than what had previously existed.
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How to cite this article:

Hasmiwati , Jamsari , Yanwirasti , Nuzulia Irawati and Dahelmi , 2015. Isolation and Characterization of Aedes aegypti Microsatellite Markers Dengue Hemorrhagic Fever Vector in West Sumatra, Indonesia. Pakistan Journal of Biological Sciences, 18: 273-278.

DOI: 10.3923/pjbs.2015.273.278

URL: https://scialert.net/abstract/?doi=pjbs.2015.273.278

 
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