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Pakistan Journal of Biological Sciences
  Year: 2015 | Volume: 18 | Issue: 3 | Page No.: 122-128
DOI: 10.3923/pjbs.2015.122.128
 
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Optimization of Split Pea and Dry Figs-Based Media for the Growth of Lactobacillus plantarum through Plackett-Burman and Response Surface Methodological Approaches
Zohra Kassas, Baida Djeghri-Hocine, Saida Hanoune, Zineb Derradji, Aicha Boudour and Messaouda Boukhemis

Abstract:
Plackett-Burman design was used to determine the most influential of 9 variables (glucose, lactulose, tween80, K2HPO4, sodium acetate, MgSO4, pH, shaking and inoculum size) on the growth of L. plantarum BH14. Glucose, lactulose, MgSO4 and shaking had a significant effect on growth of this strain at a 70% confidence level. Glucose, lactulose and MgSO4 showed positive coefficient but shaking had a negative coefficient, thus shaking was avoided in subsequent experiments. Composite design was used to determine the optimum concentrations of the significant variables. An optimized formulation of nutrition levels was suggested from the software at the following concentrations: 11.59 g L–1 glucose, 11.59 g L–1 lactulose and 0.23 g L–1 MgSO4. Growth of L plantarum was compared to that recorded on the reference medium ‘vegetal MRS’. The results showed that viable counts in the optimized culture medium were significantly higher than those in the vegetal MRS medium.
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How to cite this article:

Zohra Kassas, Baida Djeghri-Hocine, Saida Hanoune, Zineb Derradji, Aicha Boudour and Messaouda Boukhemis, 2015. Optimization of Split Pea and Dry Figs-Based Media for the Growth of Lactobacillus plantarum through Plackett-Burman and Response Surface Methodological Approaches. Pakistan Journal of Biological Sciences, 18: 122-128.

DOI: 10.3923/pjbs.2015.122.128

URL: https://scialert.net/abstract/?doi=pjbs.2015.122.128

 
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