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Ameliorating Role of Rutin on Oxidative Stress Induced by Iron Overload in Hepatic Tissue of Rats |
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Samy Ali Hussein Aziza,
Mohammed El-said Azab and Soheir Kamal El-Shall |
Abstract:
Iron is an essential element that participates in several
metabolic activities of cells; however, excess iron is a major cause of iron-induced
oxidative stress and several human diseases. Natural flavonoids, as rutin, are
well-known antioxidants and could be efficient protective agents. Therefore,
the present study was undertaken to evaluate the protective influence of rutin
supplementation to improve rat antioxidant systems against IOL-induced hepatic
oxidative stress. Sixty male albino rats were randomly divided to three equal
groups. The first group, the control, the second group, iron overload group,
the third group was used as iron overload+rutin group. Rats received six doses
of ferric hydroxide polymaltose (100 mg kg-1 b.wt.) as one dose every
two days, by intraperitoneal injections (IP) and administerated rutin (50 mg
kg-1 b.wt.) as one daily oral dose until the sacrificed day. Blood
samples for serum separation and liver tissue specimens were collected three
times, after three, four and five weeks from the onset of the experiment. Serum
iron profiles total iron, Total Iron Binding Capacity (TIBC), Unsaturated Iron
Binding Capacity (UIBC), transferrin (Tf) and Transferrin Saturation% (TS%)},
ferritin, albumin, total Protein, total cholesterol, triacylglycerols levels
and aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities
were determined. Moreover, total iron in the liver, L-malondialdehyde (L-MDA),
glutathione (GSH), Nitric Oxide (NO) and Total Nucleic Acid (TNA) levels and
glutathione peroxidase (GPx), catalase (CAT) and superoxide dismutase (SOD)
activities were also determined. The obtained results revealed that, iron overload
(IOL) resulted in significant increase in serum iron, TIBC, Tf, TS% and ferritin
levels and AST and ALT activities and also increased liver iron, L-MDA and NO
levels. Meanwhile, it decreased serum UIBC, total cholesterol, triacylglycerols,
albumin, total protein and liver GSH, TNA levels and Gpx, CAT and SOD activities
when compared with the control group. Rutin administration to iron-overloaded
rats resulted in significant decrease in serum total iron, TIBC, Tf, TS%, ferritin
levels and AST and ALT activities and liver total iron, L-MDA and NO levels
with significant increases in serum UIBC, albumin, total protein and total cholesterol
levels and in liver GSH, CAT and SOD activities compared with the IOL group.
This study provides in vivo evidence that rutin administration can improve the
antioxidant defence systems against IOL-induced hepatic oxidative stress in
rats. This protective effect in liver of iron-loaded rats may be due to both
antioxidant and metal chelation activities.
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How to cite this article:
Samy Ali Hussein Aziza, Mohammed El-said Azab and Soheir Kamal El-Shall, 2014. Ameliorating Role of Rutin on Oxidative Stress Induced by Iron Overload in Hepatic Tissue of Rats. Pakistan Journal of Biological Sciences, 17: 964-977.
DOI: 10.3923/pjbs.2014.964.977
URL: https://scialert.net/abstract/?doi=pjbs.2014.964.977
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