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Pakistan Journal of Biological Sciences
  Year: 2014 | Volume: 17 | Issue: 7 | Page No.: 931-936
DOI: 10.3923/pjbs.2014.931.936
 
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Effects of Epidermal Growth Factor, Platelet Derived Growth Factor and Growth Hormone on Cultured Rat Keratinocytes Cells in vitro

Manouchehr Safari, Laya Ghahari and M.D. Babak Hossein Zadeh Zoroufchi

Abstract:
Some growth factors, such as Epidermal Growth Factor (EGF), Growth Hormone (GH) and Platelet Derived Growth Factor (PDGF) have beneficial effects on keratinocyte proliferation and wound healing. Although the mechanism of these factors is unclear. In response to injury, growth factors are secreted by kinds of cutaneous cells. The goal of this project is to investigate the factors that could cause proliferate of the keratinocyte cells in vitro. The keratinocytes were removed from rat pups (10 days). Cultured in media with different concentration of GH, PDGF and EGF separately. The proliferation of cells was evaluated by the method of MTT and 3H-thymidine incorporation. Proliferation of keratinocytes was significantly higher in experimental groups than in control group. EGF maximally stimulated at 10 and 25 ng mL-1. PDGF-BB maximally stimulated at 50 ng mL-1, respectively. And maximal stimulation of GH was 2.5 IU L-1. GH, PDGF-BB and EGF stimulate keratinocyte cells proliferation in different concentration. These growth factors could play in healing of the skin.
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How to cite this article:

Manouchehr Safari, Laya Ghahari and M.D. Babak Hossein Zadeh Zoroufchi, 2014. Effects of Epidermal Growth Factor, Platelet Derived Growth Factor and Growth Hormone on Cultured Rat Keratinocytes Cells in vitro. Pakistan Journal of Biological Sciences, 17: 931-936.

DOI: 10.3923/pjbs.2014.931.936

URL: https://scialert.net/abstract/?doi=pjbs.2014.931.936

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