Experimental Pathological Studies of an Indian Chicken Anaemia Virus Isolate and its Detection by PCR and FAT


	Pakistan Journal of Biological Sciences
	Year: 2014 \| Volume: 17 \| Issue: 6 \| Page No.: 802-811 DOI: 10.3923/pjbs.2014.802.811

Experimental Pathological Studies of an Indian Chicken Anaemia Virus Isolate and its Detection by PCR and FAT

Mohd Yaqoob Wani, Kuldeep Dhama, Shyma K. Latheef, Rajamani Barathidassan, Ruchi Tiwari, Sandip Chakraborty, Milind Madhukar Chawak and Shambhu Dayal Singh

Abstract: Chicken Infectious Anaemia Virus (CIAV) is one of the potent immunosuppressive and economically important agents affecting poultry industry worldwide. Recent reports indicate the emergence of this virus in the poultry flocks of the country. The present study aimed to investigate the pathogenic potential of a recent isolate of CIAV obtained from poultry flock of Uttaranchal State, India. Twenty first day-old age Specific Pathogen Free (SPF) chicks were inoculated intramuscularly with 10^4.5 median tissue culture infective dose (TCID₅₀) of CIAV passaged in the Marek’s disease virus transformed chicken splenic T lymphocyte (MDCC-MSB1) cell line while 15 chicks were kept as control. The CIAV isolate produced consistent clinical signs, loss in body weight gain, anaemia, low haematocrit values, bone marrow aplasia and generalized lymphoid atrophy. Mean Packed Cell Volume (PCV) value of the infected chicks was significantly low (18.22±2.22) compared to control group (34.12±4.72) at 14 day post infection (dpi). The establishment of virus infection in chicks was confirmed both at molecular and antigenic levels by Polymerase Chain Reaction (PCR) and Indirect Immunofluorescent Test (IIFT), respectively. Characteristic apoptotic pattern was also detected in the affected organs and the virus was re-isolated successfully in MDCC-MSB1 cell cultures. The present results revealed that the virus circulating in poultry flocks of Uttaranchal state is both pathogenic and immunosuppressive in nature. Extensive epidemiological studies are suggested in the poultry flocks of the country along with adaptation of appropriate diagnostic, prevention and control strategies so as to prevent economic losses caused by this important poultry pathogen.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Novel and Emerging Therapies Safeguarding Health of Humans and Their Companion Animals: A Review

•	Immunomodulatory and Therapeutic Potentials of Herbal, Traditional/Indigenous and Ethnoveterinary Medicines

•	Chicken Infectious Anemia Status in Commercial Broiler Chickens Flocks in Assiut-upper Egypt: Occurrence, Molecular Analysis Using PCR-RFLP and Apoptosis Effect on Affected Tissues

How to cite this article:

Mohd Yaqoob Wani, Kuldeep Dhama, Shyma K. Latheef, Rajamani Barathidassan, Ruchi Tiwari, Sandip Chakraborty, Milind Madhukar Chawak and Shambhu Dayal Singh, 2014. Experimental Pathological Studies of an Indian Chicken Anaemia Virus Isolate and its Detection by PCR and FAT. Pakistan Journal of Biological Sciences, 17: 802-811.

DOI: 10.3923/pjbs.2014.802.811

URL: https://scialert.net/abstract/?doi=pjbs.2014.802.811

COMMENT ON THIS PAPER

	Full Name:
	E-mail:
	Comments:

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		AGRIS
		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		Google Scholar
		ISI Web of Knowledge
		PubMed
		SCIMAGO
		Thomson ISI

Follow @scialert

Science Alert