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Pakistan Journal of Biological Sciences
  Year: 2014 | Volume: 17 | Issue: 4 | Page No.: 597-600
DOI: 10.3923/pjbs.2014.597.600
 
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Micropropagation of Dalbergia sissoo Roxb. Through Tissue Culture Technique

Jyoti Sahu, Shagufta Khan, Ram Kumar Sahu and Amit Roy

Abstract:
Multiple shoots of Dalbergia sissoo Roxb. (Sissoo) were incited from seeds through indirect somatic embryogenesis method. Seeds were inoculated in Murashige and Skoog’s medium without any growth hormone. Than cotyledonary leaves were struck and used for callus induction on MS medium amplified with 2, 4-dichlorophenoxyacetic acid (0.5 to 4 mg mL-1). After 3 to 4 weeks the embryogenic callus clumps was transferred to medium supplemented with cytokinin (BAP 1 to 5 mg L-1, kinetin 1-5.0 mg L-1) for embryo maturation and germination. The high-frequency shoot proliferation (82%) and maximum number of shoots per explants were recorded in MS medium containing NAA (0.5)+BAP (0.5). The findings of recent investigations have shown that, it is possible to induce indirect somatic embryogenesis in Dalbergia sissoo and plant regeneration from callus cultures derived from cotyledonary leaves as explants.
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How to cite this article:

Jyoti Sahu, Shagufta Khan, Ram Kumar Sahu and Amit Roy, 2014. Micropropagation of Dalbergia sissoo Roxb. Through Tissue Culture Technique. Pakistan Journal of Biological Sciences, 17: 597-600.

DOI: 10.3923/pjbs.2014.597.600

URL: https://scialert.net/abstract/?doi=pjbs.2014.597.600

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