Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Pakistan Journal of Biological Sciences
  Year: 2014 | Volume: 17 | Issue: 4 | Page No.: 578-581
DOI: 10.3923/pjbs.2014.578.581
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Evaluation of Gastrothylax cruminifer Antigenic Preparation in Serodiagnosis of Paramphistomiasis in Sheep

Tariq Ahmad, Mohammad Latif Reshi, M.Z. Cheshti, Syed Tanveer, Zaffar Amin Shah, Bashir Ahmad Fomada and O.K Raina

Abstract:
An evaluation of Gastrothylax cruminifer crude antigen preparation viz., Somatic Antigen (SAg), Exerelory Secretary Antigen (ESAg) and Egg Antigen (EAg) in serodiagnosis of disease was undertaken. Test sera samples were obtained from 30 Paramphistomiasis Positive and 30 Gastrothylax free sheep slaughtered at Hazratbal Kashmir. The referral antigenic preparation were evaluated against Paramphistomiasis positive sera, via., control negative sera, using double immunodiffusion test (DID), (IEP) Immunoelectrophoretic assay and ELISA. The performance of referral antigens, as assessed from percent sensentivity and specificity, revealed an increasing trend from DID (Double immunodiffusion-An immunological technique used in the detection, identification and quantification of antibodies and antigens) to IEP (immunoelectrophoresis-A general name for a number of biochemical methods for separation and characterization of proteins based on electrophoresis and reaction with antibodies), followed by ELISA, detecting higher number of sheep positive for paramphistomisis. In ELISA the ESAg and SAg were evaluated as most reactive antigens with no significant difference and EAg was the least antigenic. In IEP, EAg had the higher sensitivity (60%) and analogous specificity of SAg and ESAg. The formation of the preceptin lines in the proximity to EAg containing wells (cathode end) in IEP was suggestive of higher molecular weight of G. cumnifer specific protein molecules with slower rate of migration. Purification and characterization of G. cumnifer and identification of specific antigenic molecules, particularly in EAg has been suggested for qualitative improvement of diagnostic value of the antigens in the tests used here in.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

Tariq Ahmad, Mohammad Latif Reshi, M.Z. Cheshti, Syed Tanveer, Zaffar Amin Shah, Bashir Ahmad Fomada and O.K Raina, 2014. Evaluation of Gastrothylax cruminifer Antigenic Preparation in Serodiagnosis of Paramphistomiasis in Sheep. Pakistan Journal of Biological Sciences, 17: 578-581.

DOI: 10.3923/pjbs.2014.578.581

URL: https://scialert.net/abstract/?doi=pjbs.2014.578.581

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 

Curve Bottom