
	Pakistan Journal of Biological Sciences
	Year: 2014 \| Volume: 17 \| Issue: 2 \| Page No.: 173-181 DOI: 10.3923/pjbs.2014.173.181

Isolation, Culture Optimization and Physico-chemical Characterization of Laccase Enzyme from Pleurotus fossulatus

P. Chowdhury, R. Hari, B. Chakraborty, B. Mandal, S. Naskar and Nirmalendu Das

Abstract: Pleurotus fossulatus (Cooke) Sace is member of oyster mushroom can produced extracellular laccase (benzenediol: oxygen oxidoreductase; EC 1.10.3.2) in submerged fermentation. To analyze the optimum production for laccase P. fossulatus was cultured both in stationary and shaking condition in different media. Partial purification of laccase was done after 0-80% ammonium sulphate precipitation, followed by DEAE (Diethylaminoethyl) Sephadex (A-50) anion exchange chromatography. Potato-sucrose peptone (PSP) medium and Potato-dextrose (PD) medium showed highest laccase production in shaking and stationary conditions, respectively. Though the time required for optimum laccase production in stationary condition was much more than the shaking condition but the amount of laccase was about 2.75t greater in former condition. The laccase produced in stationary condition was more stable than the enzyme produced in shaking condition. The partially purified enzyme showed highest affinity towards o-dianisidine than guaiacol and ABTS (2,2’- Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) as evidenced by their K_m. The physico-chemical properties of the laccase suggested the significance of this enzyme in industrial applications.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Experimental Evidence for Presence of a Growth Regulating Extracellular Laccase in Some Pleurotus Species

•	Microbial Laccases and their Applications: A Review

•	Nano Immobilization of Enzyme to Improvement of Biofuel Cell Electrode's Function

•	Laccase from Spent Mushroom Compost of Lentinus polychrous Lev. And its Potential for Remazol Brilliant Blue R Decolourisation

•	Development of a Simple Method for a New Immunoconjugate Utilizing Laccase

How to cite this article:

P. Chowdhury, R. Hari, B. Chakraborty, B. Mandal, S. Naskar and Nirmalendu Das, 2014. Isolation, Culture Optimization and Physico-chemical Characterization of Laccase Enzyme from Pleurotus fossulatus. Pakistan Journal of Biological Sciences, 17: 173-181.

DOI: 10.3923/pjbs.2014.173.181

URL: https://scialert.net/abstract/?doi=pjbs.2014.173.181

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		AGRIS
		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Google Scholar
		ISI Web of Knowledge
		PubMed

Science Alert