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Pakistan Journal of Biological Sciences
  Year: 2014 | Volume: 17 | Issue: 1 | Page No.: 109-113
DOI: 10.3923/pjbs.2014.109.113
Occurrence of Extended Spectrum Beta-lactamases Producing Alpha Hemolytic Escherichia coli in Neonatal Diarrhea
Amit Kumar, Amit Kumar Verma, Subhash Malik, Manoj Kumar Gupta, Arvind Sharma and Anu Rahal

Abstract:
E. coli, often highly pathogenic in neonatal and immuno-compromised patients, are usually supposed susceptible to a variety of chemotherapeutic agents, however with the time and also due to the extensive use of chemotherapeutic agents irrespective of their susceptibility, have evolved drug resistant strains. Moreover, the production of extended spectrum β-lactamases producing enteric pathogens is a serious issue. In this context the present study was conducted to find out occurrence of extended spectrum Beta-lactamases producing alpha hemolytic Escherichia coli in neonates, of less than one month of age, suffering from diarrhea. Fecal samples were collected from various private hospitals in Mathura and Agra districts of Uttar Pradesh, India. With the help of hospital nursing staffs sterilized stool samples were collected and processed for isolation of E. coli. The double disk diffusion method was applied to assess the ESBL production. E. coli organisms were isolated from 39 kids out of 120 samples. The assessment of isolates revealed alpha hemolytic nature of 23 isolates on 5% sheep blood agar. As usual when drug sensitivity was performed that revealed their multi drug resistance pattern which on further examination with double disk method showed 17 of them to be extended spectrum β-lactamases producing E. coli. The presence of enterohemorrhagic extended spectrum β-lactamases producing Escherichia coli in kids is a matter of concern and public health importance as it may be due to the transmission from hospital itself during the birth time or post birth admission period.
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How to cite this article:

Amit Kumar, Amit Kumar Verma, Subhash Malik, Manoj Kumar Gupta, Arvind Sharma and Anu Rahal, 2014. Occurrence of Extended Spectrum Beta-lactamases Producing Alpha Hemolytic Escherichia coli in Neonatal Diarrhea. Pakistan Journal of Biological Sciences, 17: 109-113.

DOI: 10.3923/pjbs.2014.109.113

URL: https://scialert.net/abstract/?doi=pjbs.2014.109.113

 
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