
	Pakistan Journal of Biological Sciences
	Year: 2014 \| Volume: 17 \| Issue: 8 \| Page No.: 1015-1022 DOI: 10.3923/pjbs.2014.1015.1022

Low Cost Medium for Spore Production of Bacillus KKU02 and KKU03 and the Effects of the Produced Spores on Growth of Giant Freshwater Prawn (Macrobrachium rosenbergii de Man)

Charkrit Wangka-Orm, Sirirat Deeseenthum and Vichai Leelavatcharamas

Abstract: In order to extend the shelf life of 2 high potential Bacillus probiotic isolates which were Bacillus KKU02 and Bacillus KKU03, the spore forms of these 2 Bacillus isolates were studied for using as probiotic instead. The low cost medium for spore production of these 2 Bacillus isolates was examined in order to produce probiotic spores for feeding the shrimps. It was found that cassava at 100 g L^-1 and supplemented with 20.0 g L^-1 dextrose, 0.1 g L^-1 MgSO₄ and 2.0 g L^-1 (NH₄)₂SO₄ showed the highest spore concentration at about 1x10⁸ CFU mL^-1. The effects of feeding these 2 Bacillus spores on the growth of giant freshwater prawns were further examined. The spores of Bacillus KKU02 and Bacillus KKU03 (~10⁷spore mL^-1) in pure and mixed culture forms were mixed with commercial prawn feed (200 mL kg^-1) to give six feed treatments. Body length and weight of the prawns in mixed spore culture tanks after rearing for 90 days (13.5 cm and 59.8 g, respectively) were significantly higher (p = 0.05) than other treatments. The treated prawns were further challenged with Aeromonas hydrophila for 7 days. The percentages of survival after the challenge in the prawns fed with the mixed spores (46.8%) were also found significantly higher (p = 0.05) than others groups, except the mixed live cell treatment (60%). These results indicated that the spores of Bacillus KKU02 and Bacillus KKU03 had a high potential for using as commercial probiotics.

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•	Effect of Feeding Bacillus sp. As Probiotic Bacteria on Growth of Giant Freshwater Prawn (Macrobrachium rosenbergii de Man)

How to cite this article:

Charkrit Wangka-Orm, Sirirat Deeseenthum and Vichai Leelavatcharamas, 2014. Low Cost Medium for Spore Production of Bacillus KKU02 and KKU03 and the Effects of the Produced Spores on Growth of Giant Freshwater Prawn (Macrobrachium rosenbergii de Man). Pakistan Journal of Biological Sciences, 17: 1015-1022.

DOI: 10.3923/pjbs.2014.1015.1022

URL: https://scialert.net/abstract/?doi=pjbs.2014.1015.1022

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