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Pakistan Journal of Biological Sciences
  Year: 2013 | Volume: 16 | Issue: 24 | Page No.: 2046-2049
DOI: 10.3923/pjbs.2013.2046.2049
 
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Alginate Immobilization of Escherichia coli MTCC 1652 Whole Cells for Bioconversion of Glycyrrhizinic acid and into 18-β Glycyrrhetinic Acid

M. Ahmad and B.P. Panda

Abstract:
Microbial biotransformation of Glycyrrhizinic acid (GL) into 18-β Glycyrrhetinic Acid (GA) was achieved using Escherichia coli MTCC 1652 whole cell. The E. coli whole cell was immobilized by entrapment method within calcium alginate beads using cell suspension of equal volume with sodium alginate 8%. The pH of solution, reaction volume and % of GL were optimized during the immobilization procedure and optimum pH 6.5, reaction volume of 4 mL and at 3% GL concentration for 12 h of incubation time showed highest concentration of GA (72.649 μg mL-1) with 76% bioconversion of GL to GA. Under optimized condition the immobilized cell produces 58.663 μg per mL of GA in licorice root extract containing 95.118 μg of GL per mL of the extract with 61% conversion at 12 h.
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How to cite this article:

M. Ahmad and B.P. Panda, 2013. Alginate Immobilization of Escherichia coli MTCC 1652 Whole Cells for Bioconversion of Glycyrrhizinic acid and into 18-β Glycyrrhetinic Acid. Pakistan Journal of Biological Sciences, 16: 2046-2049.

DOI: 10.3923/pjbs.2013.2046.2049

URL: https://scialert.net/abstract/?doi=pjbs.2013.2046.2049

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