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Pakistan Journal of Biological Sciences
  Year: 2013 | Volume: 16 | Issue: 24 | Page No.: 1929-1935
DOI: 10.3923/pjbs.2013.1929.1935
 
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Association of CTLA-4 Gene 49A/G Polymorphism with the Incidence of Type 1 Diabetes Mellitus in the Iranian Kurdish Population

Slahadin Ahmadi, Jalal Rostamzadeh, Darya Khosravi, Parvin Shariati and Nadia Shakiba

Abstract:
Cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) has an inhibitory function on T cells and is critical for the induction of peripheral tolerance. CTLA-4 +49 G allele affects the CTLA-4 function and has been reported to be correlated with a higher risk of various autoimmune diseases including type 1 diabetes (T1D). The present study was conducted to investigate the association between the polymorphism of the CTLA-4 exon 1+49 A/G and susceptibility to TID and type 2 diabetes (T2D) in Kurds living in Iranian Kurdistan. The+49 A/G polymorphism was analyzed in 60 patients with T1D, 56 patients with T2D and 107 control subjects using PCR Single-strand Conformation Polymorphism (SSCP) and restriction fragment length polymorphism methods. All studied populations (T1D, T2D and Controls) were in Hardy-Weinberg equilibrium (p, 0.39, 0.94 and 0.89, respectively). Both+49 G allele (p = 0. 015, OR = 1. 86) and +49 A/G genotype frequencies (p = 0. 012, OR = 2. 31) were significantly higher in T1D patients than control. There was significant over-representation of the G allele in female T1D patients. No significant differences in +49 G allele and +49 A/G genotype frequencies were found between T2D and control subjects. SSCP analysis did not show new mutation in the amplified segment. The results of this study indicate that CTLA-4+49 A/G gene polymorphism confers genetic susceptibility to T1D but not T2D in the Kurdish population living in Iranian Kurdistan and women carrying the +49 G allele are at greater risk of getting T1D than men having the G allele.
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How to cite this article:

Slahadin Ahmadi, Jalal Rostamzadeh, Darya Khosravi, Parvin Shariati and Nadia Shakiba, 2013. Association of CTLA-4 Gene 49A/G Polymorphism with the Incidence of Type 1 Diabetes Mellitus in the Iranian Kurdish Population. Pakistan Journal of Biological Sciences, 16: 1929-1935.

DOI: 10.3923/pjbs.2013.1929.1935

URL: https://scialert.net/abstract/?doi=pjbs.2013.1929.1935

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