Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Pakistan Journal of Biological Sciences
  Year: 2013 | Volume: 16 | Issue: 24 | Page No.: 1913-1921
DOI: 10.3923/pjbs.2013.1913.1921
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Transformation of Morinda citrifolia Via Simple Mature Seed Imbibition Method

J.J. Lee, S. Ahmad and H.A. Roslan

Abstract:
Morinda citrifolia, is a valuable medicinal plant with a wide range of therapeutic properties and extensive transformation study on this plant has yet been known. Present study was conducted to establish a simple and reliable transformation protocol for M. citrifolia utilising Agrobacterium tumefaciens via direct seed exposure. In this study, the seeds were processed by tips clipping and dried and subsequently incubated in inoculation medium. Four different parameters during the incubation such as incubation period, bacterial density, temperature and binary vectors harbouring β-glucuronidase (GUS) gene (pBI121 and pGSA1131), were tested to examine its effect on transformation efficiency. The leaves from the treated and germinated seedlings were analysed via Polymerase Chain Reaction (PCR), histochemical assay of the GUS gene and reverse transcription-PCR (RT-PCR). Results of the study showed that Agrobacterium strain LBA4404 with optical density of 1.0 and 2 h incubation period were optimum for M. citrifolia transformation. It was found that various co-cultivation temperatures tested and type of vector used did not affect the transformation efficiency. The highest transformation efficiency for M. citrifolia direct seed transformation harbouring pBI121 and pGSA1131 was determined to be 96.8% with 2 h co-cultivation treatment and 80.4% when using bacterial density of 1.0, respectively. The transformation method can be applied for future characterization study of M. citrifolia.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

J.J. Lee, S. Ahmad and H.A. Roslan, 2013. Transformation of Morinda citrifolia Via Simple Mature Seed Imbibition Method. Pakistan Journal of Biological Sciences, 16: 1913-1921.

DOI: 10.3923/pjbs.2013.1913.1921

URL: https://scialert.net/abstract/?doi=pjbs.2013.1913.1921

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 

Curve Bottom