Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Pakistan Journal of Biological Sciences
  Year: 2013 | Volume: 16 | Issue: 24 | Page No.: 1873-1883
DOI: 10.3923/pjbs.2013.1873.1883
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

The Particularities of Protein fraction in the Apoptosis of Lymphocytes of Patients with Asthma

C.A. Vodounon, Z.I. Abramova, N. Aikou, A. Sezan, S.O. Kotchoni, S.A. Akpona and Lamine Baba-Moussa

Abstract:
The emergence of various diseases specifically severe diseases such as bronchial asthma is associated with apoptosis of lymphocytes. One of the major biochemical features of apoptosis is chromosome DNA fragmentation implemented by apoptotic nucleases. The inactivation of these apoptotic nucleases produces undigested DNA and is linked to a number of autoimmune disorders. Instead of this we have studied the enzymatic activities of the cytoplasmic and nucleic proteins of lymphocytes from healthy donors and patients with bronchial asthma. The study of enzymatic activities of the nuclease of lymphocytes was assessed by flow cytometry, spectrofluorimetry and electrophoresis method in agarose gel. In the peripheral blood cells of healthy donors undergoing apoptosis, we found a DNAse activated by Ca2+ and Mg2+ ions. Lymphocytes of patients with bronchial asthma contain DNAses, the activity of which depends on the seriousness of the desease. In patients cells, the activity of the Mn2+-dependent DNAse increases, whereas the activity of the Ca2+, Mg2+-dependent DNase decreases. Taking into consideration the role of the Ca2+, Mg2+-dependent DNAse in apoptosis, we can propose that there is a link between the reduction of the rate of apoptosis of lymphocytes in patients with bronchial asthma and the dysfunction of the induction of “apoptotical” Ca2+, Mg2+-dependent nuclease. According to the results obtained, we can assume that why apoptosis of lymphocytes resist in patients with bronchial asthma is a reduction in concentration of endocellular calcium and an increase of manganese ions content, which results in the triggering of activation mechanism for Mn2+-dependent endonuclease activity. This leads to the change of DNA fragmentation nature in lymphocytes and as a consequence, to disorders in process of apoptotic bodies’ formation, thus, hindering apoptosis of lymphocytes in patients with bronchial asthma.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    The Implication of Morphological Characteristics in the Etiology of Allergic Asthma Disease and in Determining the Degree of Severity of Atopic and Bronchial Asthma
How to cite this article:

C.A. Vodounon, Z.I. Abramova, N. Aikou, A. Sezan, S.O. Kotchoni, S.A. Akpona and Lamine Baba-Moussa, 2013. The Particularities of Protein fraction in the Apoptosis of Lymphocytes of Patients with Asthma. Pakistan Journal of Biological Sciences, 16: 1873-1883.

DOI: 10.3923/pjbs.2013.1873.1883

URL: https://scialert.net/abstract/?doi=pjbs.2013.1873.1883

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 

Curve Bottom