• [email protected]
  • +971 507 888 742
Submit Manuscript
SciAlert
  • Home
  • Journals
  • Information
    • For Authors
    • For Referees
    • For Librarian
    • For Societies
  • Contact
  1. Pakistan Journal of Biological Sciences
  2. Vol 15 (8), 2012
  3. 374-379
  • Online First
  • Current Issue
  • Previous Issues
  • More Information
    Aims and Scope Editorial Board Guide to Authors Article Processing Charges
    Submit a Manuscript

Pakistan Journal of Biological Sciences

Year: 2012 | Volume: 15 | Issue: 8 | Page No.: 374-379
DOI: 10.3923/pjbs.2012.374.379

Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend



Total views 366

Search


Authors


S.M. Nourazarian

Country: Iran

G. Irajian

Country: Iran

M. Najafi

Country: Iran

M. Nourbakhsh

Country: Iran

J. Maleki

Country: Iran

M. Shabani

Country: Iran

Keywords


  • oxidative stress
  • nitric oxide
  • diabetic nephropathy
  • Lactobacillus reuteri
  • bone morphogenetic protein-7
  • diabetic rats
  • transforming growth factor beta
Research Article

The Effect of Lactobacillus reuteri on Bone Morphogenetic Protein-7 and Beta Transforming Growth Factor Gene Expressions in Streptozotocin-induced Diabetic Rat’s Kidneys

S.M. Nourazarian, G. Irajian, M. Najafi, M. Nourbakhsh, J. Maleki and M. Shabani
Diabetes mellitus is a serious health problem in the world and about 20 to 40% of the patients are being affected with diabetic nephropathy. The anti diabetic property of Lactobacillus reuteri (L. reuteri) has been reported. The study designed to investigate the effect of L. reuteri on the expression of BMP-7 and TGF-β genes, the two basic factors involved in diabetic nephropathy. This experimental study was carried out in two months. For this goal thirty male Wistar rats with 12 weeks old and 200±50 g weight was divided into 5 groups, each consisting six rats. (1) Non diabetic, (2) Untreated diabetic, (3) Diabetic rats fed with L. reuteri, (4) Diabetic rats treated with insulin (4-5 U/kg/day), (5) Non diabetic rat fed with L. reuteri. Diabetes in the was induced single intraperitoneal (i.p.) injection of streptozotocin (50 mg kg-1 b. wt). The L. reuteri 1x108 Colony Forming Units (CFU) were administered via oral gavages. After two months rats were anesthetized and blood samples collected. Serum nitric oxide (NO) levels were determined by a chemiluminescence method using NO analyzer and serum glucose by glucose oxidize method. The expression of BMP-7 and TGF-β genes in the rat’s kidneys were determined by real time PCR. Results showed that BMP-7 gene expression was increased in diabetic rats that fed with L. reuteri, while TGF-β gene expressions were decreased. Histopathological study showed that administration of L. reuteri (1x108 CFU/rat/day) significantly reduced kidney fibrosis and increased meaningfully NO levels in diabetic rats fed with L. reuteri. It was concluded that L. reuteri increase BMP-7 gene expression and may prevents from renal damage by oxidative stress by increasing antioxidant capacity.
PDF Fulltext XML References Citation

How to cite this article

S.M. Nourazarian, G. Irajian, M. Najafi, M. Nourbakhsh, J. Maleki and M. Shabani, 2012. The Effect of Lactobacillus reuteri on Bone Morphogenetic Protein-7 and Beta Transforming Growth Factor Gene Expressions in Streptozotocin-induced Diabetic Rat’s Kidneys. Pakistan Journal of Biological Sciences, 15: 374-379.

DOI: 10.3923/pjbs.2012.374.379

URL: https://scialert.net/abstract/?doi=pjbs.2012.374.379

Leave a Comment


Your email address will not be published. Required fields are marked *

Useful Links

  • Journals
  • For Authors
  • For Referees
  • For Librarian
  • For Socities

Contact Us

Office Number 1128,
Tamani Arts Building,
Business Bay,
Deira, Dubai, UAE

Phone: +971 507 888 742
Email: [email protected]

About Science Alert

Science Alert is a technology platform and service provider for scholarly publishers, helping them to publish and distribute their content online. We provide a range of services, including hosting, design, and digital marketing, as well as analytics and other tools to help publishers understand their audience and optimize their content. Science Alert works with a wide variety of publishers, including academic societies, universities, and commercial publishers.

Follow Us
© Copyright Science Alert. All Rights Reserved