
	Pakistan Journal of Biological Sciences
	Year: 2012 \| Volume: 15 \| Issue: 3 \| Page No.: 152-155 DOI: 10.3923/pjbs.2012.152.155

Phytochemical Investigation and in vitro Antinociceptive Activity of Clerodendrum indicum Leaves

Sheikh Zahir Raihan, Pranoyjit Biswas, Md. Moniruzzaman Monir, Subrata Kumar Biswas, Anusua Chowdhury and A.K.M. Shahidur Rahman

Abstract: The crude ethanolic extracts of Clerodendrum indicum Linn. leaves were investigated for possible antinociceptive activity using acetic acid induced writhing model in mice. Phytochemical analysis was also carried out according to the standard procedures to identify the presence of different phytoconstituents in the ethanolic extract of the plant leaves. The study results showed 38.91 and 55.24% inhibition of writhings in the tested mice when ethanolic extract of Clerodendrum indicum Linn. leaves at doses of 250 and 500 mg kg^-1 body weight was given intraperitoneally, respectively. The study results were also compared with antinociceptive activity of the standard drug, Diclofenac sodium (68.37% inhibition) used at 25 mg kg^-1 body weight. At the above doses, the crude ethanolic extract of the plant showed significant antinociceptive activity in dose dependent fashion in acetic acid-induced writhing model in mice. The inhibition of writhings was calculated in respective to control group and it was found that p-values (<0.0001) obtained in all cases were extremely statistically significant. However, the phytochemical analysis showed the presence of alkaloid, steroid, saponin, tannin, reducing sugar and gum. The results suggest that crude ethanolic extracts of Clerodendrum indicum leaves possess significant antinociceptive properties justifying its folkloric use as analgesics and further research is necessary to isolate the principle phytochemical constituent(s) responsible for this activity.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Mechanism of the Anti-inflammatory Activity of Khaya senegalensis A. Juss. (Meliaceae)

•	Antinociceptive, Antiinflammatory and Antibacterial Properties of Tamarix indica Roots

•	Evaluation of Antinociceptive and Antidiarrhoeal Properties of Pistia stratiotes (Araceae) Leaves

•	In vitro Antibacterial Principles of Extracts and Two Flavonoids from Clerodendrum indicum Linn

•	Antinociceptive and Anti-inflammatory Properties of Melastoma malabathricum Leaves Chloroform Extract in Experimental Animals

How to cite this article:

Sheikh Zahir Raihan, Pranoyjit Biswas, Md. Moniruzzaman Monir, Subrata Kumar Biswas, Anusua Chowdhury and A.K.M. Shahidur Rahman, 2012. Phytochemical Investigation and in vitro Antinociceptive Activity of Clerodendrum indicum Leaves. Pakistan Journal of Biological Sciences, 15: 152-155.

DOI: 10.3923/pjbs.2012.152.155

URL: https://scialert.net/abstract/?doi=pjbs.2012.152.155

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		AGRIS
		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		Google Scholar
		ISI Web of Knowledge
		PubMed

Science Alert