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Pakistan Journal of Biological Sciences
  Year: 2011 | Volume: 14 | Issue: 9 | Page No.: 533-539
DOI: 10.3923/pjbs.2011.533.539
 
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Optimization of Temperature, Moisture Content and Inoculum Size in Solid State Fermentation to Enhance Mannanase Production by Aspergillus terreus SUK-1 using RSM

Jahwarhar Izuan Abdul Rashid, Noraini Samat and Wan Mohtar Wan Yusoff

Abstract:
Optimization of three parameters, temperature (25-35°C), moisture content (40% (w/v)-60% (w/v) and inoculum sizes (5% (w/v)-15% (w/v) were investigated and optimized by Response Surface Methodology (RSM) for optimal mannanase production by Aspergillus terreus SUK-1. A second order polynomial equation was fitted and the optimum condition was established. The result showed that the moisture content was a critical factor in terms of its effect on mannanase. The optimum condition for mannanase production was predicted at 42.86% (w/v) initial moisture (31°C) temperature and 5.5% (w/v) inoculum size . The predicted optimal parameter were tested in the laboratory and the mannanase activity 45.12 IU mL-1 were recorded to be closed to the predicted value (44.80 IU mL-1). Under the optimized SSF condition (31°C, 42.86% moisture content (w/v) and 5.5% inoculum size (w/v)), the maximum mannanase production was to prevail about 45.12 IU mL-1 compare to before optimized (30°C, 50% moisture content (w/v) and 10% inoculum size (w/v)) was only 34.42 IU mL-1.
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 RELATED ARTICLES:
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How to cite this article:

Jahwarhar Izuan Abdul Rashid, Noraini Samat and Wan Mohtar Wan Yusoff, 2011. Optimization of Temperature, Moisture Content and Inoculum Size in Solid State Fermentation to Enhance Mannanase Production by Aspergillus terreus SUK-1 using RSM. Pakistan Journal of Biological Sciences, 14: 533-539.

DOI: 10.3923/pjbs.2011.533.539

URL: https://scialert.net/abstract/?doi=pjbs.2011.533.539

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