
	Pakistan Journal of Biological Sciences
	Year: 2011 \| Volume: 14 \| Issue: 23 \| Page No.: 1038-1046 DOI: 10.3923/pjbs.2011.1038.1046

Influence of Nigella sativa Fixed Oil on Some Blood Parameters and Histopathology of Skin in Staphylococcal-Infected BALB/c Mice

Mariam A. Abu- Al-Basal

Abstract: Nigella sativa has been used for a long time in Jordanian folk medicine to treat skin diseases like microbial infections and inflammation. Therefore, the present study was conducted to assess the healing efficacy of petroleum ether extract of Nigella sativa seeds (fixed oil) on staphylococcal-infected skin. Male BALB/c mice were infected with 100 μL of Staphylococcus aureus (ATCC 6538) suspension at a dose of 10⁸colony forming unit/mouse into shaved mild dorsal skin. Application of treatments for each group (100 μL sterile saline, 100 μL chloramphenicol (10 μg/mouse) and Nigella sativa fixed oil at a dose of 50, 100 or 150 μL/mouse) was performed at the site of infection twice a day for two consecutive days after 3 h of infection. At day 3 and 5 after infection, total White Blood Cells (WBCs) count; differential and absolute differential WBC counts and the number of viable bacteria present in the skin area were measured. At day 5 after infection, the animals were sacrificed and the histology of skin was examined. Results indicated that fixed oil of Nigella sativa seeds enhance healing of staphylococcal-infected skin by reducing total and absolute differential WBC counts, local infection and inflammation, bacterial expansion and tissue impairment. These effects provide scientific basis for the use of Nigella sativa in traditional medicine to treat skin infections and inflammations.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Protective Effects of Ethanolic Extract of Nigella sativa Seed in Paracetamol Induced Acute Hepatotoxicity In vivo

•	Histological Evaluation of the Healing Properties of Dead Sea Black Mud on Full-thickness Excision Cutaneous Wounds in BALB/c Mice

How to cite this article:

Mariam A. Abu- Al-Basal , 2011. Influence of Nigella sativa Fixed Oil on Some Blood Parameters and Histopathology of Skin in Staphylococcal-Infected BALB/c Mice. Pakistan Journal of Biological Sciences, 14: 1038-1046.

DOI: 10.3923/pjbs.2011.1038.1046

URL: https://scialert.net/abstract/?doi=pjbs.2011.1038.1046

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		AGRIS
		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		Google Scholar
		ISI Web of Knowledge
		PubMed
		SCIMAGO
		Thomson ISI

Science Alert