• [email protected]
  • +971 507 888 742
Submit Manuscript
SciAlert
  • Home
  • Journals
  • Information
    • For Authors
    • For Referees
    • For Librarian
    • For Societies
  • Contact
  1. Pakistan Journal of Biological Sciences
  2. Vol 14 (23), 2011
  3. 1038-1046
  • Online First
  • Current Issue
  • Previous Issues
  • More Information
    Aims and Scope Editorial Board Guide to Authors Article Processing Charges
    Submit a Manuscript

Pakistan Journal of Biological Sciences

Year: 2011 | Volume: 14 | Issue: 23 | Page No.: 1038-1046
DOI: 10.3923/pjbs.2011.1038.1046

Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend



Total views 457

Authors


Mariam A. Abu- Al-Basal

Country: Jordan

Keywords


  • cutaneous infection
  • hematology
  • Staphylococcus aureus
  • Nigella sativa
  • histological analysis
Research Article

Influence of Nigella sativa Fixed Oil on Some Blood Parameters and Histopathology of Skin in Staphylococcal-Infected BALB/c Mice

Mariam A. Abu- Al-Basal
Nigella sativa has been used for a long time in Jordanian folk medicine to treat skin diseases like microbial infections and inflammation. Therefore, the present study was conducted to assess the healing efficacy of petroleum ether extract of Nigella sativa seeds (fixed oil) on staphylococcal-infected skin. Male BALB/c mice were infected with 100 μL of Staphylococcus aureus (ATCC 6538) suspension at a dose of 108 colony forming unit/mouse into shaved mild dorsal skin. Application of treatments for each group (100 μL sterile saline, 100 μL chloramphenicol (10 μg/mouse) and Nigella sativa fixed oil at a dose of 50, 100 or 150 μL/mouse) was performed at the site of infection twice a day for two consecutive days after 3 h of infection. At day 3 and 5 after infection, total White Blood Cells (WBCs) count; differential and absolute differential WBC counts and the number of viable bacteria present in the skin area were measured. At day 5 after infection, the animals were sacrificed and the histology of skin was examined. Results indicated that fixed oil of Nigella sativa seeds enhance healing of staphylococcal-infected skin by reducing total and absolute differential WBC counts, local infection and inflammation, bacterial expansion and tissue impairment. These effects provide scientific basis for the use of Nigella sativa in traditional medicine to treat skin infections and inflammations.
PDF Fulltext XML References Citation

How to cite this article

Mariam A. Abu- Al-Basal, 2011. Influence of Nigella sativa Fixed Oil on Some Blood Parameters and Histopathology of Skin in Staphylococcal-Infected BALB/c Mice. Pakistan Journal of Biological Sciences, 14: 1038-1046.

DOI: 10.3923/pjbs.2011.1038.1046

URL: https://scialert.net/abstract/?doi=pjbs.2011.1038.1046

Related Articles

Protective Effects of Ethanolic Extract of Nigella sativa Seed in Paracetamol Induced Acute Hepatotoxicity In vivo
Histological Evaluation of the Healing Properties of Dead Sea Black Mud on Full-thickness Excision Cutaneous Wounds in BALB/c Mice

Leave a Comment


Your email address will not be published. Required fields are marked *

Useful Links

  • Journals
  • For Authors
  • For Referees
  • For Librarian
  • For Socities

Contact Us

Office Number 1128,
Tamani Arts Building,
Business Bay,
Deira, Dubai, UAE

Phone: +971 507 888 742
Email: [email protected]

About Science Alert

Science Alert is a technology platform and service provider for scholarly publishers, helping them to publish and distribute their content online. We provide a range of services, including hosting, design, and digital marketing, as well as analytics and other tools to help publishers understand their audience and optimize their content. Science Alert works with a wide variety of publishers, including academic societies, universities, and commercial publishers.

Follow Us
© Copyright Science Alert. All Rights Reserved