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Pakistan Journal of Biological Sciences
  Year: 2010 | Volume: 13 | Issue: 22 | Page No.: 1092-1098
DOI: 10.3923/pjbs.2010.1092.1098
In vitro Antioxidant, Lipoxygenase and Xanthine Oxidase Inhibitory Activities of Fractions from Cienfuegosia digitata Cav., Sida alba L. and Sida acuta Burn f. (Malvaceae)
K. Konate, A. Souza, A.Y. Coulibaly, N.T.R. Meda, M. Kiendrebeogo, A. Lamien-Meda, J. Millogo-Rasolodimby, M. Lamidi and O.G. Nacoulma

Abstract:
In this study polyphenol content, antioxidant activity, lipoxygenase (LOX) and Xanthine Oxidase (XO) inhibitory effects of n-hexane, dichloromethane, ethyl acetate and n-butanol fractions of aqueous acetone extracts from S. alba L., S. acuta Burn f. and Cienfuegosia digitata Cav. were investigated. The total phenolics, flavonoids, flavonols and total tannins were determined by spectrophotometric methods using Folin-ciocalteu, AlCl3 reagents and tannic acid, respectively. The antioxidant potential was evaluated using three methods: inhibition of free radical 2,2-diphenyl-1-picrylhydramzyl (DPPH), ABTS radical cation decolorization assay and Iron (III) to iron (II) reduction activity (FRAP). For enzymatic activity, lipoxygenase and xanthine oxidase inhibitory activities were used. This study shows a relationship between polyphenol contents, antioxidant and enzymatic activities. Present results showed that ethyl acetate and dichloromethane fractions elicit the highest polyphenol content, antioxidant and enzymatic activities.
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How to cite this article:

K. Konate, A. Souza, A.Y. Coulibaly, N.T.R. Meda, M. Kiendrebeogo, A. Lamien-Meda, J. Millogo-Rasolodimby, M. Lamidi and O.G. Nacoulma, 2010. In vitro Antioxidant, Lipoxygenase and Xanthine Oxidase Inhibitory Activities of Fractions from Cienfuegosia digitata Cav., Sida alba L. and Sida acuta Burn f. (Malvaceae). Pakistan Journal of Biological Sciences, 13: 1092-1098.

DOI: 10.3923/pjbs.2010.1092.1098

URL: https://scialert.net/abstract/?doi=pjbs.2010.1092.1098

 
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