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Pakistan Journal of Biological Sciences
  Year: 2009 | Volume: 12 | Issue: 10 | Page No.: 765-771
DOI: 10.3923/pjbs.2009.765.771
 
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The Protective Effects of Garlic Extract Against Acetaminophen-Induced Oxidative Stress and Glutathione Depletion

M. Anoush, M.A. Eghbal, F. Fathiazad, H. Hamzeiy and N.S. Kouzehkonani

Abstract:
Acetaminophen, the most commonly sold over-the-counter antipyretic analgesic, is capable of causing severe and sometimes fatal hepatic damage in humans and experimental animals. The incidence of liver injury due to acetaminophen overdose, either with suicidal intent or by accident, is increasing. Garlic is among those medicinal plants famous for its different health protective effects. In this study, the protective effects of garlic extract on acute acetaminophen-induced liver injury were investigated using freshly isolated rat hepatocytes. The hepatocytes were isolated from Sprague-Dawley male rats by a two step collagenase model. Formation of Reactive Oxygen Species (ROS) and Glutathione (GSH) depletion were studied after addition of acetaminophen to cell suspensions. The effects of garlic extract on prevention of ROS formation as well as GSH depletion was investigated and compared with the effects of N-Acetyl Cysteine (NAC) as the standard treatment. Reactive oxygen species formation was assessed by a spectrofluorometry method and garlic extract was shown to be as effective as NAC in decreasing ROS formation induced by acetaminophen. Glutathione (GSH) levels of hepatocytes were determined using HPLC. Garlic extract was effective in preventing GSH depletion significantly (p<0.05). It is concluded that garlic extract has an antioxidant effect and can protect hepatocytes from GSH depletion following NAPQI production.
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How to cite this article:

M. Anoush, M.A. Eghbal, F. Fathiazad, H. Hamzeiy and N.S. Kouzehkonani, 2009. The Protective Effects of Garlic Extract Against Acetaminophen-Induced Oxidative Stress and Glutathione Depletion. Pakistan Journal of Biological Sciences, 12: 765-771.

DOI: 10.3923/pjbs.2009.765.771

URL: https://scialert.net/abstract/?doi=pjbs.2009.765.771

COMMENTS
30 May, 2009
Fakour Ahmadiani:
This is an acceptible paper in PJBS. I will be glad to send papers to PJBS with the qualified papers like this one
06 June, 2009
abd akrom:
good, qualified and applicable for us
03 August, 2009
sadollah mohammadi:
I studied this basic and experimental article. In my opinion, it is a useful and applied research in this simple but life- threatening subject about Acetaminophen toxicity.and ,I hope ,with continuity trying and indefatigable preservance of article authors and other researchers , for solving an important problem at the medical and therapeutics events ,the results of this research could enter as Pharmaceutical Products to the drug marketing in near future
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