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Pakistan Journal of Biological Sciences
  Year: 2009 | Volume: 12 | Issue: 24 | Page No.: 1564-1570
DOI: 10.3923/pjbs.2009.1564.1570
 
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In vitro Antioxidative Activities and Polyphenol Content of Eugenia polyantha Wight Grown in Indonesia

Raden Arthur Ario Lelono, Sanro Tachibana and Kazutaka Itoh

Abstract:
Antioxidative activities of three bark extracts (methanol, methanol-water and water) from Eugenia polyantha Wight grown in Indonesia were evaluated using various in vitro assays; 2, 2-diphenyl-1-picrylhydrazyl radical-scavenging, hydrogen peroxide-scavenging and β-carotene bleaching assays. From the assays, Eugenia polyantha bark extracts were found to be potential antioxidative activities. The methanol-water extract showed the highest level of free radical-scavenging activity (Effective dose (ED50) = 0.18 mg mL-1) and protection from β-carotene bleaching (85.7% at 100 μg mL-1). The methanol-water extracts showed the highest total phenolic content (856 mg gallic acid equivalent (GAE)/g and 161 mg catechin equivalent (CE)/g) and total antioxidative capacity (449 mg ascorbic acid equivalent (AAE)/g). Furthermore, it exhibited dose-dependent antioxidative activities. A relationship between total antioxidative capacity and total phenolic content was recognized in the three extracts from Eugenia polyantha bark.
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How to cite this article:

Raden Arthur Ario Lelono, Sanro Tachibana and Kazutaka Itoh, 2009. In vitro Antioxidative Activities and Polyphenol Content of Eugenia polyantha Wight Grown in Indonesia. Pakistan Journal of Biological Sciences, 12: 1564-1570.

DOI: 10.3923/pjbs.2009.1564.1570

URL: https://scialert.net/abstract/?doi=pjbs.2009.1564.1570

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