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Pakistan Journal of Biological Sciences
  Year: 2009 | Volume: 12 | Issue: 18 | Page No.: 1246-1252
DOI: 10.3923/pjbs.2009.1246.1252
 
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Standardization and Assessment of Cell Culture Media Quantities in Roller Poly Ethylene Terephthalate Bottles Employed in the Industrial Rabies Viral Vaccine Production

S. Jagannathan, S. Chaansha, K. Rajesh, T. Santhiya, C. Charles and K.N. Venkataramana

Abstract:
Vero cells are utilized for production of rabies vaccine. This study deals with the optimize quantity media require for the rabies vaccine production in the smooth roller surface. The rabies virus (Pasteur vaccine strain) is infected to monolayer of the various experimented bottles. To analyze the optimal quantity of media for the production of rabies viral harvest during the process of Vero cell derived rabies vaccine. The trials are started from 200 to 400 mL (PTARV-1, PTARV-2, PTARV-3, PTARV-4 and PTARV-5). The samples are taken in an appropriate time intervals for analysis of In Process Quality Control (IPQC) tests. The collected viral harvests are further processed to rabies vaccine in a pilot level and in addition to scale up an industrial level. Based on the evaluation the PTARV-2 (250 mL) show highly encouraging results for the Vero cell derived rabies vaccine production.
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How to cite this article:

S. Jagannathan, S. Chaansha, K. Rajesh, T. Santhiya, C. Charles and K.N. Venkataramana, 2009. Standardization and Assessment of Cell Culture Media Quantities in Roller Poly Ethylene Terephthalate Bottles Employed in the Industrial Rabies Viral Vaccine Production. Pakistan Journal of Biological Sciences, 12: 1246-1252.

DOI: 10.3923/pjbs.2009.1246.1252

URL: https://scialert.net/abstract/?doi=pjbs.2009.1246.1252

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