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Pakistan Journal of Biological Sciences
  Year: 2008 | Volume: 11 | Issue: 1 | Page No.: 86-91
DOI: 10.3923/pjbs.2008.86.91
 
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Method and Quantity of Liquid Formulation of Phosphobacteria Required for Seed Inoculation

M. Gomathy, M. Thangaraju, S. Gunasekaran, N.O. Gopal and H. Gopal

Abstract:

The survival of vegetative and sporulated cells of the Bacillus cultures on the seeds of the crop plants was tried in different combinations. One milliliter inoculum with 1 mL adhesive combination or sterile water showed better results followed by 1.5 mL inoculum with 0.5 mL adhesive or sterile water. The population of 5.5x105 cfu seed-1 on black gram, 10.5x105 cfu seed-1 on soybean and 6.5x105 cfu seed-1 on maize were observed after 12 h of incubation in 1 mL sporulated inoculum mixed with 1 mL of rice gruel. The sporulated inoculum along with rice gruel favoured the adherence of the regenerated cells as rice gruel is rich in nutrient content.

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How to cite this article:

M. Gomathy, M. Thangaraju, S. Gunasekaran, N.O. Gopal and H. Gopal, 2008. Method and Quantity of Liquid Formulation of Phosphobacteria Required for Seed Inoculation. Pakistan Journal of Biological Sciences, 11: 86-91.

DOI: 10.3923/pjbs.2008.86.91

URL: https://scialert.net/abstract/?doi=pjbs.2008.86.91

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