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Pakistan Journal of Biological Sciences
  Year: 2008 | Volume: 11 | Issue: 5 | Page No.: 778-782
DOI: 10.3923/pjbs.2008.778.782
 
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Plant Regeneration from Cell Suspension Culture of Potato(Solanum tuberosum L.)

Firozeh Torabi, Ahmad Majad and Ali Akbar Ehsanpour

Abstract:
In the present study, for callus production leaf and stem segments of potato cultivar White Desiree were cultured on MS medium supplemented with 2,4-D, NAA and Kinetin (callus production medium). Calli then were transferred in the same liquid medium for cell suspension production. In the next step cell suspensions were transferred back to the callus production medium. Finally, calli derived from cell suspension were cultured on 6 different shoot initiation media (S1-S6). However, on S6 medium with combination of GA3 and BAP more than 80% of the calli produced shoot buds and shoots. Fully grown shoots then were rooted and produced whole plants. Chromosome and morphological analysis showed no somaclonal variation among regenerated plants.
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How to cite this article:

Firozeh Torabi, Ahmad Majad and Ali Akbar Ehsanpour, 2008. Plant Regeneration from Cell Suspension Culture of Potato(Solanum tuberosum L.). Pakistan Journal of Biological Sciences, 11: 778-782.

DOI: 10.3923/pjbs.2008.778.782

URL: https://scialert.net/abstract/?doi=pjbs.2008.778.782

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