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Pakistan Journal of Biological Sciences
  Year: 2008 | Volume: 11 | Issue: 1 | Page No.: 62-67
DOI: 10.3923/pjbs.2008.62.67
 
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Evaluation of Dyes Decolourisation by the Crude Enzyme from Pleurotus sajor-caju Grown on Sorghum Seed Media

R. Sarnthima and S. Khammuang

Abstract:

The extracellular enzymes from Pleurotus sajor-caju were studied for lignin degrading enzyme patterns and dye decolourisation potential. Laccases are major ligninolytic enzymes excreted by the fungus. The results from a native-PAGE revealed that there were at least two isoenzymes. The crude enzyme had a pH and a temperature optimum at 6.0 and 40°C, respectively when 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) was used as substrate. The pH and thermal stability were at 5.0 and 30°C. The pH optima for decolourisation of Indigo Carmine and Methyl Red were at 5.0 and 6.0, respectively. Indigo Carmine could be decolorized efficiently above 90% within 180 min, whereas Methyl Red could be decolorized only 3.5%. High efficiency decolourisation of Indigo Carmine makes this fungus to be a promise choice in biological treatment of waste water containing Indigo Carmine.

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How to cite this article:

R. Sarnthima and S. Khammuang, 2008. Evaluation of Dyes Decolourisation by the Crude Enzyme from Pleurotus sajor-caju Grown on Sorghum Seed Media. Pakistan Journal of Biological Sciences, 11: 62-67.

DOI: 10.3923/pjbs.2008.62.67

URL: https://scialert.net/abstract/?doi=pjbs.2008.62.67

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