Characteristics, Efficacy and Safety Testing of Standardized Extract of Croton tiglium Seed from Indonesia as Laxative Material

Research Article

**Characteristics, Efficacy and Safety Testing of Standardized Extract of Croton tiglium Seed from Indonesia as Laxative Material**

Saputera, Djumali Mangunwidjaja, Sapta Raharja, Leonardus B.S. Kardono and Dyah Iswantini

Identification and taxonomy analysis conducted at Herbarium Bogoriense at Research Centre for Biology, Indonesian Institute of Sciences Bogor. The name of the plant was C. tiglium L. The result of analysis on C. tiglium, ethanol extract as laxative material using the intestinal transit method showed treatment group that received dosage 0.06 mL/30 g b.wt. (72.5%) was significantly different compared to negative control (48.4%) or positive control (50.6%) which showed the weak effect as laxative at the dosage of 0.75 mL/30 g b.wt. It showed that ethanol extract of C. tiglium seed at dosage 0.06 mL/30 g is effective as laxative. The test result of the treatment using dosage 0.06, 0.04, 0.026 and 0.07 mL/28 g of body weight showed the mice population response 100, 60, 40 and 40% consecutively. The Thompson and Weil analysis result showed the ED₅₀ was at 0.027 mL or equal to 639,5 g kg^-1 b.wt. The LD₅₀ was at 0.0707 equals with 1674,5 mg kg^-1 b.wt. Safety limit is the range of dosage that cause the lethal effect and the dosage that gives the intended effect. The safety limit is represented by the comparison of LD₅₀/ED₅₀. Calculation result that the extract safety limit was LD₅₀/ED₅₀ = 0.0707/0.027 = 2.7.

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Saputera, Djumali Mangunwidjaja, Sapta Raharja, Leonardus B.S. Kardono and Dyah Iswantini, 2008. Characteristics, Efficacy and Safety Testing of Standardized Extract of Croton tiglium Seed from Indonesia as Laxative Material. Pakistan Journal of Biological Sciences, 11: 618-622.

DOI: 10.3923/pjbs.2008.618.622

URL: https://scialert.net/abstract/?doi=pjbs.2008.618.622

Pakistan Journal of Biological Sciences

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Authors

Saputera

Djumali Mangunwidjaja

Sapta Raharja

Leonardus B.S. Kardono

Dyah Iswantini

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Research Article

**Characteristics, Efficacy and Safety Testing of Standardized Extract of Croton tiglium Seed from Indonesia as Laxative Material**

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