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Pakistan Journal of Biological Sciences
  Year: 2008 | Volume: 11 | Issue: 4 | Page No.: 613-617
DOI: 10.3923/pjbs.2008.613.617
 
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Obtaining of a Symbiotic Product Based on Lactic Bacteria, Pollen and Honey

Vamanu Adrian, Vamanu Emanuel, Popa Ovidiu, Campeanu Gheorghe, Albulescu Radu, Drugulescu Manuel, Nita Sultana and Babeani Narcisa

Abstract:
This study presents the evolution of a Lactobacillus plantarum 2s strain and of a Lactobacillus acidophilus 1a strain on media containing pollen and honey. The following parameters were determined: CFU value, lactic acid production, sugar consumption and presentation of the final product. The researches were performed during 72 h, in tightly closed recipients, at a temperature of 37 °C, containing various quantities of milled or non-milled pollen (P1: 20 g non-milled pollen, 3 g honey, 5 mL distilled water; P2: 20 g milled pollen, 3 g honey, 5 mL distilled water; P3: 20% non-milled pollen, 3% honey; P4: 20% milled pollen, 3% honey). The media were very well homogenized before inoculation. The inoculation was made only after the medium gained a homogenous consistency. The inoculum consists of a 48 h culture of Lactobacillus plantarum 2s and Lactobacillus acidophilus, on LE medium, in equal proportions. The testing of the nutritive value of the symbiotic product was made on wistar rats, males and females, divided into lots of 10 animals each. The animals were administered symbiotic product every day, in their food, in intakes of 2 mg kg-1 (lot I), 20 mg kg-1 (lot II) and 200 mg kg-1 (lot III), compared to a control. During the 4 weeks of the experiment, no lethality cases were recorded in any of lots, or in control. The animals involved in the experiment were examined daily and did not present changes of appetite, of behavior or clinical signs of disease.
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How to cite this article:

Vamanu Adrian, Vamanu Emanuel, Popa Ovidiu, Campeanu Gheorghe, Albulescu Radu, Drugulescu Manuel, Nita Sultana and Babeani Narcisa, 2008. Obtaining of a Symbiotic Product Based on Lactic Bacteria, Pollen and Honey. Pakistan Journal of Biological Sciences, 11: 613-617.

DOI: 10.3923/pjbs.2008.613.617

URL: https://scialert.net/abstract/?doi=pjbs.2008.613.617

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