
	Pakistan Journal of Biological Sciences
	Year: 2008 \| Volume: 11 \| Issue: 24 \| Page No.: 2653-2659 DOI: 10.3923/pjbs.2008.2653.2659

Tetraploid Induction of Hyoscyamus muticus L. using Colchicine Treatment

F. Shahriari-Ahmadi, E. Dehghan, M. Farsi and M. Azizi

Abstract: In this study the tip of meristem in diploid plants (2n = 2x =28) were treated with a 0.2% colchicine solution and flowcytometry was used to determine the DNA ploidy level of different tissues in individual plants. Morphological characteristics such as size and thickness of leaves, flowers, seeds, epidermis and guard cells, as well as the number of chloroplast in guard cells were considered as other parameters for identifying putative tetraploid plants. We show that, the DNA ploidy of Hyoscyamus muticus, treated with Colchicine can be evaluated using flow cytometry. In addition we also report reliable morphological markers to identify putative tetraploids in this species. Although, tetraploidy was induced using this technique, mixoploid plants were also observed among the Egyptian henbane plants. The results of this study also indicate that colchicine treatment can efficiently be used to improve Hyoscyamus muticus for commercial and pharmaceutical applications of its vegetative organs.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Development of the Flow Cytometric Protocol for Ploidy Analysis and Determination of Relative Nuclear DNA Content in Cashew (Anacardium occidentale Linn.)

How to cite this article:

F. Shahriari-Ahmadi, E. Dehghan, M. Farsi and M. Azizi, 2008. Tetraploid Induction of Hyoscyamus muticus L. using Colchicine Treatment. Pakistan Journal of Biological Sciences, 11: 2653-2659.

DOI: 10.3923/pjbs.2008.2653.2659

URL: https://scialert.net/abstract/?doi=pjbs.2008.2653.2659

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		AGRIS
		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		Google Scholar
		ISI Web of Knowledge
		PubMed
		SCIMAGO
		Thomson ISI

Science Alert